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相关概念视频

Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Real Time RT-PCR02:57

Real Time RT-PCR

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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相关实验视频

Updated: Sep 9, 2025

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
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Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

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基共价探针用于RNA

Jinwoo Shin, Moon Jung Kim, Eric T Kool

    bioRxiv : the preprint server for biology
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    此摘要是机器生成的。

    研究人员开发了一种新的共价光标记方法,为细胞中RNA的成像和跟踪提供高选择性和亮度. 这种进步改善了现有的RNA染料,使得更清晰的可视化和分析.

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    科学领域:

    • 生物化学
    • 分子生物学
    • 化学生物学

    背景情况:

    • 目前的非共价RNA染料缺乏对RNA与DNA的选择性.
    • 现有的染料在细胞成像中表现出弱结合和高背景信号.
    • 需要改进RNA特定的光探针.

    研究的目的:

    • 为RNA开发一种新的,独立于序列的光标记策略.
    • 创建一种具有增强选择性和信号放大性的共价标签方法.
    • 在各种生物环境中实现波长调节,高准确度的RNA成像.

    主要方法:

    • 在供体-接受体和RNA的2'-基组之间利用了阿西利米达介导的反应.
    • 在温和的水性条件下对RNA进行共价附着的设计反应探针.
    • 研究了对RNA与DNA的共价探针的光增强和选择性.

    主要成果:

    • 在RNA标记时达到高达390倍的光增强.
    • 与DNA相比,对RNA的选择性高达970倍.
    • 记录了四种不同的发射颜色用于多功能成像应用.
    • 在凝,溶液和活细胞中成功应用共价基平台进行RNA分析.

    结论:

    • 开发的化共价标签方法为RNA检测提供了强大而有选择性的方法.
    • 这种平台比现有的RNA染料提供了显著的改进,使先进的RNA成像和分析成为可能.
    • 在复杂的生物系统中,共价光体系统是研究RNA的有价值的新工具.