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相关概念视频

RNA Interference01:23

RNA Interference

26.4K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
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Types of RNA01:23

Types of RNA

64.7K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
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RNA Editing02:23

RNA Editing

9.2K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.2K
RNA Stability01:53

RNA Stability

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Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
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相关实验视频

Updated: Sep 9, 2025

Novel RNA-Binding Proteins Isolation by the RaPID Methodology
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Novel RNA-Binding Proteins Isolation by the RaPID Methodology

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通过ADARs结合RNA可以防止RNA干扰攻击自身产生的dRNA

Nabeel S Ganem, Dean Light, Roni Haas

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    概括
    此摘要是机器生成的。

    作用于RNA的腺胺酶 (ADARs) 阻止RNA干扰 (RNAi) 途径准自身RNA. 通过阻止siRNA生成,ADARs对抗RNAi,保护宿主转录物免受免疫识别和降解.

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    相关实验视频

    Last Updated: Sep 9, 2025

    Novel RNA-Binding Proteins Isolation by the RaPID Methodology
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    Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
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    Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
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    科学领域:

    • 分子生物学
    • 免疫学
    • 遗传学

    背景情况:

    • 有机体区分自身和外来RNA以调节免疫反应并避免自身免疫.
    • 双链RNA (dsRNA) 是免疫反应的关键触发器,但也存在于宿主细胞中.
    • A-to-I RNA编辑 (通过ADARs) 和RNA干扰 (RNAi) 是dSRNA调节和基因表达的关键宿主途径.

    研究的目的:

    • 研究ADARs和RNAi在调节dRNA中的相互作用.
    • 确定ADAR如何阻止免疫系统向自身RNA.
    • 在体内检查ADARs在防止外源RNAi中的作用.

    主要方法:

    • 在模型生物Caenorhabditis elegans中研究了siRNA结构和数量.
    • 使用ADAR突变动物来评估对siRNA生成的影响.
    • 研究了ADARs在体内防止外源RNAi的能力.

    主要成果:

    • ADAR突变显示显著增加了针对编辑基因的siRNAs.
    • ADARs对抗RNAi处理的初始步骤,防止从编辑部位生成初级siRNA.
    • ADARs干扰外源RNAi的疗效,可能会阻止转静止,与ADR- 2结合有关.

    结论:

    • 在保护自身产生的dRNA免受异常免疫识别方面,ADARs起着至关重要的作用.
    • 通过ADARs调节的RNA编辑过程防止自我RNA引发不必要的免疫反应.
    • ADARs作为一个关键的检查点,区分自身的dsRNA与外来的dsRNA,以维持平衡.