菌素Rho因子的相分离与酸性压力有关
bioRxiv : the preprint server for biology
|September 5, 2025
概括
具有独特的额外区域的mycobacterial Rho蛋白经历液-液相分离 (LLPS) 形成细胞凝聚物. 这种由内在无序区域驱动的LLPS机制对于细菌的应激耐受性至关重要.
科学领域:
- 细菌转录的调节
- 蛋白质生物物理学
- 微生物应激反应
背景情况:
- Rho是一种细菌转录终止因子.
- 一些菌株Rho蛋白具有独特的额外区域.
- 这些区域预计本质上是无序的,并且涉及液态相分离 (LLPS).
研究的目的:
- 为了研究菌根Rho蛋白中的LLPS.
- 确定额外区域在Rho介导的LLPS中的作用.
- 探索Rho LLPS在真菌菌中的相关性.
主要方法:
- 来自真菌菌种的Rho蛋白的生物信息分析.
- 在实验室描述Rho凝结物形成.
- 在* Mycolicibacterium smegmatis*中观察Rho相分离
- 基因操纵Rho以评估其他区域的功能.
主要成果:
- 来自* Mycolicibacterium smegmatis* 的Rho在体外和体内通过LLPS形成凝结物.
- 这种LLPS取决于菌根Rho中存在的其他区域.
- 仅仅是内在无序的区域就足以在体外形成液滴.
- 在*M. smegmatis*中,在酸性应激过程中发生Rho相分离,影响细胞适应性.
结论:
- LLPS是一种广泛用于将菌根Rho组装成功能凝聚物的机制.
- 额外的Rho区域对于其相隔性质至关重要.
- 通过Rho介导的LLPS可能在调节转录和使菌根体能承受压力方面发挥作用.
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