通过下一代测序实验样本来解码丰富图书馆的Aptamer特异性
bioRxiv : the preprint server for biology
|September 5, 2025
概括
一种新的方法,APTAMER特异性测序以实现有效向 (ASSET),直接量化APTAMER特异性. 这加快了用于治疗和诊断的真实阳性体的发现.
科学领域:
- 生物技术
- 分子生物学
- 基因组学
背景情况:
- 在SELEX过程中,通过测序选择回合识别RNA和DNA体.
- 目前的方法从丰富模式推断出aptamer特异性,缺乏直接量化.
研究的目的:
- 引入Aptamer特异性测序以实现有效向 (ASSET),以直接量化aptamer特异性.
- 为了使得APTAMER能够被准确地分类为真假阳性/阴性.
主要方法:
- ASSET使用下一代测序 (NGS) 与内部参考序列进行规范化.
- 它计算出具有统计意义的aptamer特异性得分,并生成特异性概况.
- 将ASSET分数与传统的SELEX测序数据进行整合.
主要成果:
- 资产特异性得分与实验测量特异性有很强的相关性.
- 该方法可以有效地将真正阳性和虚假阳性分离出来.
- 与丰富度或丰富度指标相比,ASSET的表现更为出色.
结论:
- 在SELEX之后,ASSET提供了更有效的测量标准来选择性胺基候选物.
- 这种框架加快了治疗和诊断应用的aptamer发现.
- 通过ASSET,提高了适合物识别的可靠性和效率.
相关概念视频
03:21RNA-seq
10.4K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.4K
03:00Next-generation Sequencing
92.5K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
92.5K