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在Cichorium intybus中确定第一个植物咖啡基因酸酶

Antoine Mallavergne¹, David Mathiron², Roland Molinié³

¹Joint Laboratory CHIC41H University of Lille-Florimond-Desprez, Université de Picardie Jules Verne, Université de Liège, Univ. Lille, Junia, UMRT 1158 BioEcoAgro - Specialized Metabolites of Plant Origin, Villeneuve d'Ascq, France.

Frontiers in plant science

|September 5, 2025

概括

两种香草酶,CiCQE1和CiCQE3,将基酸 (5-CQA) 和异基酸 (3,5-DiCQA) 化为咖啡酸 (CA). 这表明了植物中5-CQA重新调动的新途径.

科学领域:

植物生物化学
分子生物学
代谢途径

背景情况:

酸 (5-CQA) 是咖啡酸的,在植物中大量存在,并参与应激防御.
由于共享的途径中间体,5-CQA在素形成中的潜在作用是假设的,但没有直接的酶.
识别处理5-CQA的酶对于理解其代谢命运和植物防御机制至关重要.

研究的目的:

鉴定和描述参与酸代谢的植物酶 (5-CQA).
研究这些酶在素生物合成或其他代谢途径中的潜在作用.
探索GDSL酶/脂酶家族酶在5-CQA转换中的功能.

主要方法:

在 (Cichorium intybus) 中研究的酶参与了5-CQA到3,5-DiCQA的转化.
确定了两个GDSL酶/脂酶家族的酶,即CiCQE1和CiCQE3.
在烟草中进行生物化学表征和功能分析,包括基因表达分析.

主要成果:

在体外和植物中,CiCQE1和CiCQE3有效地将5- CQA和3,5- DiCQA化为咖啡酸 (CA).
对CiCQE1和CiCQE3的基因在西瓜根中高度表达,其中5-CQA和3,5-DiCQA积累.
烟草中的短暂表达导致咖啡酸和咖啡酸的积累,这表明CA转移到其他途径.

结论:

在根中,CiCQE1和CiCQE3代表了酸 (5-CQA) 再调动的新途径.
释放的咖啡酸 (CA) 可以转化为咖啡-CoA,从而可能促进单质醇的生物合成.
需要使用突变系进行进一步的研究,以充分阐明这些酶的植物功能.

关键词:

一个GDSL 咖啡酸咖啡伊尔-普特瑞辛西瓜基酸烟草

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