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Electron Microscope Tomography and Single-particle Reconstruction

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

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Atomically Traceable Nanostructure Fabrication

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通过原子分辨率显微镜可视化的纳米级异构体中的随机接口形成

Miyuki Hanazawa¹, Masaya Sakakibara¹, Ryo Ishikawa²

¹Department of Chemistry, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.

Journal of the American Chemical Society

|September 5, 2025

概括

不混合盐的异构形成是可逆的,而不是单向的. 在现场电子显微镜揭示了动态,随机过程在环境条件下控制自我组装和异质表达.

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科学领域:

材料科学
表面科学
纳米技术

背景情况:

不同结构具有独特的特性, 推动技术创新.
异面接口的形成通常被视为一个单向的,不可逆转的过程.

研究的目的:

从纳米尺度上的不混合盐中可视化和理解异构结构形成的动态机制.
研究异构界面形成的可逆性和随机性.

主要方法:

在位电子显微镜在298K.
在环境条件下观察化 (NaCl) 和化 (NaI) 的纳米混合物.

主要成果:

观察到自我组装和异质表达是可逆的,由随机平衡控制.
自组合涉及有序和半有序状态之间的波动,由离子相互作用驱动.
表皮生长显示出重复的层生长和剥皮周期,具有低能量的屏障.

结论:

不同界面的形成是一个动态和随机的过程,而不是严格的单向或不可逆转的.
这些发现为理解高接口能系统的接口启动提供了框架.
可逆性为设计和控制异构性质提供了新的可能性.