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在单基分辨率的芯片上进行多重酶DNA合成的电子控制的脱保护化学

Lihuan Zhao¹, Qinzhuo Sun¹, Jian-Qiao Jiang²

¹State Key Laboratory of Radio Frequency Heterogeneous Integration, Shanghai Jiao Tong University, Shanghai 200240, China. xinzhao@sjtu.edu.cn.

|September 5, 2025

概括

这项研究引入了酶性DNA合成的电子控制的脱保护化学,使单链DNA (ssDNA) 的精确,高吞吐量生产具有高精度.

科学领域:

生物技术
合成生物学
材料科学

背景情况:

酶性DNA合成 (EDS) 为化学DNA合成提供了一个环保的替代方案,用于产生长且复杂的DNA序列.
当前的EDS方法在实现高吞吐量时的精确序列控制方面面临挑战.
开发自动化和可扩展的DNA制造技术对于各种生物应用至关重要.

研究的目的:

开发一种新的高通量多重化酶 DNA 合成平台,并精确控制序列.
将电子控制的脱保护化学 (ECDC) 与自动化DNA合成的芯片上的水凝初始化系统集成在一起.
展示开发的单链DNA生产平台的可行性和准确性.

主要方法:

开发一个将ECDC与水凝原料修改策略相结合的芯片系统.
使用电化学生成的酸 (HNO2) 来选择性地去除DNA原料上的3'-oxyamino组.
在芯片上实现多像素合成阵列,以实现单基分辨率的并行DNA合成.

主要成果:

通过ECDC平台,可以精确地控制酶的DNA合成过程.
验证实验表明单序合成的准确性为100%,双序合成的平均准确性为96%.
该系统在芯片上实现了单基分辨率的SSDNA并行合成.

结论:

新型ECDC集成平台为高通量ssDNA合成提供了高度准确的解决方案.
这项技术为可扩展和自动化酶 DNA 制造奠定了基础.
开发的系统推进了下一代DNA合成技术领域.

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