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在商业使用的马雷克病疫苗病毒中保存和明显表达圆形RNA

Camille Ponsard¹, Alexis S Chasseur^1,2, Pierre Lombard¹

¹Namur Research Institute for Life Sciences (NARILIS), Integrated Veterinary Research Unit (URVI), University of Namur, Namur, Belgium.

The Journal of general virology

|September 5, 2025

概括

在马雷克病病毒 (GaHV-2) 和相关的禽类阿尔法疹病毒中发现了循环RNA. 这些病毒circRNA可能在病毒生命周期和传播中发挥作用.

科学领域:

病毒学
分子生物学
核糖核酸生物学

背景情况:

循环RNAs (circRNAs) 越来越多地被认为在各种生物过程中的作用.
异常的circRNA表达与癌症有关,它们在病毒感染中的作用是一个新兴的研究领域.
马雷克病病毒 (Gallid alpha 2, GaHV-2) 是家禽的重要病原体,之前已经在毒性菌株中发现了circRNA.

研究的目的:

在GaHV-2感染的不同阶段识别新型病毒circRNA位点.
调查常用的马雷克病疫苗中的病毒circRNA特征,包括毒性GaHV-2,GaHV-3和MeHV-1.
描述circRNAs在病毒生命周期和禽类alphaherpesvirus传播中的作用.

主要方法:

在GaHV-2中对病毒性基因和延迟相关转录 (LAT) 的circRNA进行分类.
从保存位置识别病毒circRNA表达,包括对ICP4的反意义区域和编码DNA包装蛋白,在不同的鸟类alphaherpesvirus中.
来自毒性和毒性GaHV-2菌株的circRNA的比较分析,重点是meq位点.

主要成果:

在GaHV-2感染中发现了新的病毒性circRNA位点.
在GaHV-2,GaHV-3和MeHV-1中鉴定出一种保存的病毒circRNA表达模式,该模式源于与ICP4对抗的区域,并编码DNA包装蛋白.
在毒性GaHV-2菌株和毒性GaHV-2菌株之间观察到不同的circRNA表达水平.

结论:

在相关的禽类疹病毒中存在病毒衍生的circRNAs的保存模式.
这些保存的病毒circRNA可能有助于病毒循环的完成,并促进病毒的传播.
对病毒circRNA的进一步研究可能会揭示控制马雷克病和相关病毒感染的新目标.

关键词:

在DNA包装中马雷克的疾病圆形的RNA 疹病毒延迟时间疫苗的使用

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