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Updated: Sep 8, 2025

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
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Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

Published on: December 10, 2012

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使用基于点击化学的生物化进行全基因组复制时间分析的协议

Deniz Gökbuget1, Kayla Lenshoek1, Robert Blelloch1

  • 1The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Center for Reproductive Sciences, University of California, San Francisco, San Francisco, CA, USA; Department of Urology, University of California, San Francisco, San Francisco, CA, USA; Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, San Francisco, CA, USA.

STAR protocols
|September 5, 2025
PubMed
概括

我们开发了BioRepli-seq, 这是一种在增殖细胞中绘制全基因组DNA复制时间的新方法. 这种技术使用生物化和下一代测序来准确地定位RT.

关键词:
细胞生物学遗传学基因组学分子生物学

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Lagging Strand Synthesis01:59

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During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...
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Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
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