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Updated: Sep 8, 2025

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具有液态和高稳定性的超冷冷TEM

Emily Rennich1,2, Suk Hyun Sung1,3, Nishkarsh Agarwal3

  • 1The Rowland Institute at Harvard, Harvard University, Cambridge, MA 02138.

Proceedings of the National Academy of Sciences of the United States of America
|September 5, 2025
PubMed
概括

研究人员开发了一种用于传输电子显微镜的新型超冷液体样本持有器. 这种稳定,连续的冷流量仪器可以在25K以下的温度下进行原子分辨率成像.

关键词:
化电磁场化TEM电子显微镜液态

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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