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绘制马匹miRNA景观:用于注释,量化和可视化表达的集成管道和浏览器

Jonah N Cullen¹, Jakub Cieslak², Jessica L Petersen³

¹Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota, United States of America.

|September 5, 2025

概括

我们创建了分析马的微RNA (miRNA) 和它们的变体 (isomiR) 的工具. 这提供了最大的马类miRNA表达图谱,有助于基因调节和疾病的研究.

科学领域:

基因组学和生物信息学
分子生物学与遗传学

背景情况:

微RNAs (miRNAs) 和异构Rs是关键的基因调节剂,但对于像马这样的非模型物种的数据很少.
对马类发育和疾病中的miRNA和isomiR作用的有限理解阻碍了生物见解.

研究的目的:

开发可访问的工具来识别和量化非模型生物中的miRNA和isomiR.
创建迄今为止最全面的马类miRNA表达图谱.
促进马类功能基因组学和精准兽医研究.

主要方法:

开发了FARmiR (miRNA分析和精制框架),这是一个开源的miRNA和同位素R分析管道.
创建了AIMEE (动物同位素和MiRNA表达式探索器),这是一个交互式浏览器,用于用户友好的数据访问.
整合了来自不同马体组织和来源的461个小RNA-seq数据集.

主要成果:

预测了5,781个独特的马类miRNAs和isomiRs.
建立了最大的系统策划的马匹miRNA表达图谱.
证明了FARmiR和AIMEE对于全面的小RNA分析的有用性.

结论:

开发的工具和马类图谱显著推进了非模型物种的miRNA和isomiR生物学研究.
这种资源将加速生物标志物的发现,并增强对组织特异性基因调节的理解.
促进马类功能基因组学和个性化兽医的未来研究.

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...