核化温度和基乙对冰晶生长的影响:在极端温度波动期间对细胞活力的影响
Nishaka William1, Anika Rahman1, Jason P Acker2
1Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada.
Journal of thermal biology
|September 5, 2025
概括
在冷保存细胞运输过程中的温度波动会损害质量. 优化冷保护剂和核化温度可以提高细胞对这些变暖事件的抵抗力.
科学领域:
- 低温生物学
- 细胞生物学
- 生物技术
背景情况:
- 冷保存的细胞产品在处理和运输过程中对温度波动敏感.
- 这些温度偏差可以影响产品质量,而不仅仅是持续时间和高峰温度指标.
- 在重新加热过程中冰晶的生长是影响细胞存活的关键因素.
研究的目的:
- 研究短暂变暖事件对冷保存细胞的影响.
- 评估冷保护剂的组成和冰核温度如何影响冰晶的生长和细胞活力.
- 确定在储存和运输过程中增强细胞对温度变化的弹性.
主要方法:
- 使用冷显微镜追踪五个TWE期间的冰晶生长,峰值温度不同 (-30°C,-20°C,-10°C).
- 通过添加基乙醇粉 (HES) 或降低冰核温度来改变结条件.
- 在带血单元中记录温度概况以模拟现实条件.
主要成果:
- 最大的冰晶面积增长发生在回暖温度达到-10°C时.
- 基乙 (HES) 加速了再结晶,但在一次TWE后为Jurkat细胞提供了保护.
- 降低核化温度提高了细胞在-20°C的活力,不论HES是否存在.
结论:
- 在短暂的回暖过程中,冰晶的生长并不是伤害的唯一原因; 其他依赖温度的应力也参与其中.
- 优化冷保护剂配方和核化温度可以显著提高细胞弹性.
- 这些发现有助于减少冷治疗药物在运输过程中的质量损失.
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