长链脂肪酸通过线粒体β氧化促进了解后野猪精子中的ATP产生
Hongyan Zhang1, Shanpeng Wang1, Qi Wang1
1College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China.
Animal reproduction science
|September 5, 2025
概括
用长链脂肪酸 (LCFA) 补充野猪精液可以改善化后的精子质量. 外源性LCFA通过线粒体β氧化增强精子的运动性,活力和ATP产生,克服冷保存的局限性.
科学领域:
- 生殖生物学
- 精子冷保存
- 动物生物技术
背景情况:
- 在化后恢复精子质量方面,野猪精液冷保存存在局限性.
- 精子的运动性和活力是受冷保存影响的关键参数.
- 线粒体功能和ATP生成对于精子能量代谢至关重要.
研究的目的:
- 调查化后的猪精是否可以利用外源性长链脂肪酸 (LCFA) 来产生ATP.
- 为了确定LCFAs是否可以增强线性运动,并改善溶解后的精子活力.
- 阐明线粒体β氧化在野猪精子中LCFA利用中的作用.
主要方法:
- 猪精液被冷保存在不同度的脂质混合物 (LM) 中 (0, 0. 01%, 0. 1%, 1%).
- 评估了解后的精子参数:运动性,活力,体完整性,线粒体膜潜力 (MMP),ATP水平和酶活性 (MDH,SDH,CPT-1).
- 使用埃托莫西尔抑制了线粒体β氧化,以确认LCFA利用途径.
主要成果:
- 0. 1% LM 补充剂显著改善了解后的精子运动性,活力和体完整性.
- 这种度增加了MMP,ATP水平以及MDH,SDH和CPT1的活性.
- 埃托莫西尔治疗减弱了LCFA的有益作用,证实了它们通过β氧化在ATP生成中的作用.
结论:
- 解后的野猪精子可以吸收外源性LCFA.
- 促进线粒体β氧化,增强TCA循环活动和ATP的产生.
- 在冷保存后,补充LCFA是一种有前途的策略来改善猪精子质量.
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