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莲花种子 (Nelumbo nucifera) 蛋白质衍生的结合:隔离,特征和骨质效应

Jasmadi¹, Jae-Young Je²

¹Department of Food and Nutrition, Pukyong National University, Busan 48513, Republic of Korea; National Research and Innovation Agency, Research Center for Food Technology and Processing, Gunungkidul 55861, Indonesia.

International journal of biological macromolecules

|September 5, 2025

概括

莲花种子形成一个促进骨健康的复合体 (LSPIH-Ca). 这种复合物增强骨质细胞的分化并改善的吸收,为新营养药提供了潜力.

科学领域:

生物化学和分子生物学
营养学科学
生物材料科学

背景情况:

在营养药中探索天然蛋白质衍生的健康益处.
的生物可用性对骨健康至关重要,目前正在研究有效的输送系统.

研究的目的:

从莲花种子生产和描述结合 (LSPIH).
评估LSPIH-Ca复合物的骨质效应和运输增强.
阐明LSPIH-Ca骨质生成活性的分子机制.

主要方法:

使用光谱技术 (UV,FT-IR,拉曼,EDS) 和SEM生产和表征结合.
对来自小鼠骨髓的骨质干细胞 (D1 MSC) 的骨质效应评估,测量性酸酶活性和矿化.
研究Caco-2细胞中的运输.
分子路径 (MAPK) 和骨质标记物的分析 (BMP-2/4,Runx2,ALP,COLI).

主要成果:

莲花种子具有显著的结合能力 (49.14微克/毫克).
LSPIH- Ca复合体显示骨质细胞分化和矿化增强 (123. 29%在100μg/ ml下).
LSPIH-Ca激活了MAPK通路并提高了关键的骨质生成标志物,改善了Caco-2细胞中的运输.

结论:

莲花种子衍生的形成有效的复合物 (LSPIH-Ca),具有强大的骨质生成性.
该LSPIH-Ca复合物增强的生物可用性和吸收,这表明其在功能性食品和营养药中具有实用性.
这项研究为开发改善骨健康和整体健康的新添加剂提供了基础.

关键词:

结合运输莲花种子蛋白骨质生成

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