相关实验视频

Updated: Sep 8, 2025

Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia

Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia

Published on: March 5, 2018

在NPM1突变急性髓性白血病的残留性疾病

Pejman Hamedi-Asl¹, Amineh Hosseinkhani², Nafiseh Sanei-Ataabadi³

¹Department of Hematology and Blood Banking, School of Allied Medical Sciences, Iran University of Medical, Tehran, Iran; Research Center for Noncommunicable Diseases, Jahrom University of Medical Sciences, Jahrom, Iran.

Clinica chimica acta; international journal of clinical chemistry

|September 5, 2025

概括

在NPM1突变急性髓性白血病 (AML) 中监测可测剩余疾病 (MRD) 需要先进的分子技术. 使用qPCR,NGS,ddPCR,CRISPR,单细胞测序和AI等方法定制策略对于改善临床决策至关重要.

科学领域:

血液学和瘤学
分子诊断
生物技术

背景情况:

急性髓性白血病 (AML) 是一种基因多样性的癌症,NPM1突变是关键的生物标志物.
NPM1突变显著影响AML的诊断,预后和治疗选择.
监测可测量的残留性疾病 (MRD) 对于管理NPM1突变的AML至关重要,但缺乏标准化测试.

研究的目的:

对NPM1突变AML中MRD检测的现有和新兴分子技术进行审查.
评估各种诊断和预后工具的敏感性,特异性和临床实用性.
探索将人工智能等先进技术整合到临床实践中的潜力和挑战.

主要方法:

用于检测MRD的定量PCR (qPCR),下一代测序 (NGS) 和滴滴数字PCR (ddPCR) 的评估.
评估包括CRISPR-Cas9和单细胞测序在内的复杂AML异质性的创新方法.
分析人工智能 (AI) 和机器学习 (ML) 在诊断,预后和个性化治疗中的应用.

主要成果:

目前的分子技术为MRD检测提供了不同程度的灵敏度和特异性.
像单细胞测序和CRISPR这样的先进方法在表征克隆多样性方面具有前景.
人工智能/反洗钱工具具有提高反洗钱管理的巨大潜力,但面临着重大整合挑战.

结论:

针对NPM1突变的AML进行量身定制的MRD监测策略是必不可少的,考虑到个别的突变特征.
为了优化临床决策,需要结合包括人工智能在内的既定和新型分子技术.
克服数据质量,基础设施和监管方面的挑战对于反洗钱先进技术的广泛采用至关重要.

关键词:

人工智能 (AI)克里斯普尔-卡斯9系统滴滴数字PCR (ddPCR) 方法可测量的残留疾病 (MRD)下一代测序 (NGS)核胺 (NPM1)

更多相关视频

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Published on: September 20, 2016

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Published on: June 6, 2025

相关概念视频

Mismatch Repair

Mismatch Repair

Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...

Treatment Resistant Cancers

Treatment Resistant Cancers

Cancer is the second leading cause of death in the United States. A cancer cell is genetically unstable and hence can mutate faster. They can also modify their microenvironment and escape immune surveillance. The difficulties in treating cancer are further compounded by the emergence of rapid resistance to anticancer drugs. The most common ways to attain resistance in cancer cells include alteration in drug transport and metabolism, modification of drug target, elevated DNA damage response, or...