一种由hsa_circ_0068626编码的新型蛋白通过p62/Keap1/Nrf2信号通路介导的铁导致与年龄相关的白内障
在PubMed上查看摘要
概括
此摘要是机器生成的。在与年龄相关的白内障期间,像circTFRC这样的循环RNAs (circRNAs) 在透镜上皮细胞中促进铁. 它的新型蛋白,circTFRC-236aa,激活导致细胞损伤和潜在的治疗点的途径.
科学领域
- 眼科 眼科
- 分子生物学
- 细胞生物学
背景情况
- 与年龄相关的白内障 (ARC) 是导致视力受损的主要原因.
- 透镜上皮细胞 (LEC) 对于保持透镜透明度至关重要.
- 循环RNAs (circRNAs) 和它们编码的蛋白质在ARC病变发生过程中的作用在很大程度上尚未被探索.
研究的目的
- 在ARC过程中调查circRNAs在LEC铁的作用.
- 确定参与ARC病变的特定circRNA.
- 阐明circRNAs在LEC中调节铁的机制.
主要方法
- 在ARC中识别差异表达的circRNA序列.
- 细胞检测包括增殖,活力和铁化测量 (ROS,Fe2+水平).
- 分子分析,如FISH,多元体分析,西斑和传输电子显微镜.
主要成果
- hsa_circ_0068626 (circTFRC) 在ARC上调并局部化到LEC的细胞质中.
- 环TFRC耗尽降低了LEC活力,而过度表达导致了铁死.
- 这种新蛋白激活了p62/Keap1/Nrf2通路,抑制了GPX4并促进了铁死.
结论
- 在与年龄相关的白内障中,circTFRC充当了亲铁的调节剂.
- 编码的circTFRC-236aa蛋白通过激活p62/Keap1/Nrf2通路来驱动ARC的进展.
- 在ARC中,circTFRC是缓解LEC损伤的潜在治疗点.
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