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Yan Bai¹, Meng Li¹, Qingfen Wang¹

¹Southwest Landscape Architecture Engineering Research Center of National Forestry and Grassland Administration, College of Landscape Architecture and Horticulture Science, Southwest Forestry University, Kunming 650224, China.

Plant & cell physiology

|September 6, 2025

概括

茶叶植物

科学领域:

植物分子生物学
植物应激生理学
生物化学

背景情况:

WRKY转录因子是植物防御反应的关键调节者.
了解茶叶植物中CsWRKY15等特定的WRKY基因是提高作物弹性的关键.
茶叶植物与栗子交叉种植可以影响植物的应激反应.

研究的目的:

研究茶叶植物中同源基因WRKY15 (CsWRKY15) 的功能.
阐明控制CsWRKY15表达的调节机制.
评估CsWRKY15在赋予生物和非生物应激耐受性的作用.

主要方法:

从茶叶植物中分离和描述CsWRKY15基因及其促进物.
在各种压力条件下的基因表达分析 (ethephon,PEG,低温,GA3).
产生和分析过度表达CsWRKY15的转基因烟草植物.
使用转录因子结合部位 (LTR,GARE,MBS) 的促进体分析以及与CsHAT5的相互作用研究.

主要成果:

CsWRKY15的表达是由以太,PEG,低温以及显著的吉伯雷利克酸 (GA3) 诱导的.
烟草中CsWRKY15的过度表达通过增强抗氧化活性和上调与压力相关的基因,提高了对生物和非生物压力的耐受性.
转基因烟草在压力下表现出改善的生理指标和物理障碍.
茶叶植物的家庭主体-氨酸拉链蛋白CsHAT5与CsWRKY15的促进元件 (LTR,GARE,MBS) 结合,激活其表达.

结论:

在植物的生物和非生物应激反应中,CsWRKY15起着积极的调节作用.
吉伯酸 (GA) 生物合成和信号正调节CsWRKY15的表达.
抗氧化酶和防御基因的 CsWRKY15 激活有助于提高茶叶植物的自身抵抗力,特别是在杂种条件下.

关键词:

在CSHAT5 其他:美国自动电阻活动主办人茶叶植物与栗子交叉种植

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