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Updated: Sep 8, 2025

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Published on: April 10, 2018

基于竞争性内源性RNA的免疫相关的长非编码RNA的鉴定

Yuexuan Xu¹, Lingxian Liu¹, Yinquan Chen¹

¹Intelligent Systems and Optimization Integration, School of Automation, Guangdong University of Technology, Guangzhou, China.

International dental journal

|September 6, 2025

概括

这项研究确定CARD8-AS1是脉中关键的免疫相关的长非编码RNA (lncRNA). CARD8-AS1及其ceRNA网络对于免疫反应的调节至关重要,并可作为诊断标记物.

科学领域:

口腔生物学
免疫学
生物信息学
分子生物学

背景情况:

牙炎是一种影响牙健康的普遍炎症性口腔疾病.
了解分子机制,特别是免疫失调, 对于治疗肺炎至关重要.
长非编码RNAs (lncRNAs) 在炎症疾病中的作用越来越被认可.

研究的目的:

通过生物信息学方法识别出与免疫相关的 lncRNA.
通过竞争的内源性RNA (ceRNA) 网络阐明这些lncRNA的功能作用.
根据与免疫相关的 lncRNA 探索肺炎的潜在诊断生物标志物.

主要方法:

来自患者和健康牙纸样本的 lncRNA 的差异表达分析.
用于分类模型开发的 LASSO 回归.
单样基因组丰富分析 (ssGSEA) 和权重基因相关网络分析 (WGCNA) 以确定与免疫相关的lncRNA及其功能.
使用miRanda,TargetScan和miRDB数据库构建一个lncRNA-miRNA-mRNA网络.

主要成果:

建立了一个5-lncRNA分类模型; 确定CARD8-AS1和LINC00924具有显著的免疫相关性.
WGCNA表示CARD8-AS1和LINC00924与免疫丰富模块相关.
建立了一个以CARD8-AS1为中心的ceRNA网络,突出显示了与参与JAK-STAT信号传递的免疫相关基因 (PTGS2,NLRP3,CXCL3) 的相互作用.

结论:

lncRNA CARD8- AS1及其相关的 ceRNA 网络在肌肉炎期间调节免疫反应方面发挥着重要作用.
免疫相关的 lncRNAs,特别是 CARD8-AS1, 显示出作为 pulpitis 的新型非侵入性诊断标记物的潜力.
对CARD8-AS1的调控功能进行进一步的研究可以为皮炎提供新的治疗点.

关键词:

卡德8-AS1 具有竞争力的内源性RNA 免疫性长非编码RNA 讲台

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