相关实验视频

Updated: Sep 8, 2025

Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow

Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow

Published on: April 18, 2019

食品中可靠和敏感的超短链和多化基物质 (PFAS) 分析:极相逆相色谱方法

Consolato Schiavone¹, Francesco Romaniello¹, Paola Brizio²

¹Istituto Nazionale di Ricerca Metrologica (INRIM), Strada delle cacce 91, Turin, 10135, Italy.

Journal of chromatography. A

|September 6, 2025

概括

一种新的HPLC-MS/MS方法可以准确地检测番茄产品中的超短链基和多基物质 (USC-PFAS). 在所有样本中发现了 perfluorobutanoic acid (PFBA),这突显了污染风险和需要更好的食品安全分析.

科学领域:

环境化学
分析化学
食品科学

背景情况:

和多基物质 (PFAS),特别是超短链 (USC) 化合物,是具有潜在健康影响的持续性环境污染物.
在像番茄产品这样的复杂食品矩阵中量化极端USC-PFAS存在重大分析挑战.
现有的分析方法对这些极性化合物的灵敏度和矩阵干扰有所困难.

研究的目的:

开发和验证一种新型的高性能液体染色学与双重质谱仪 (HPLC-MS/MS) 方法相结合.
准确确定各种以番茄为基础的食品中的超短链PFAS (C1-C4).
解决复杂食品矩阵中USC-PFAS监测的分析缺口.

主要方法:

使用极性分析列和延迟列来增强保留和减少干扰.
采用同位素稀释量化,以获得高准确度和精度.
针对特定的USC-PFAS进行分析,包括二乙酸 (DFA),三乙酸 (TFA), perfluoropropanoic 酸 (PFPrA), perfluorobutanoic 酸 (PFBA) 和硫化 PFAS (PFMeS,PFEtS,PFPrS,PFBS).

主要成果:

开发的HPLC-MS/MS方法显示出极好的线性 (R2 ≥0.99),恢复率 (65-135%) 和精度 (RSD<10%).
在所有测试的番茄产品中检测到了 perfluorobutanoic acid (PFBA),其度在0.056~0.265μg/kg之间.
该方法有效地减轻了系统干扰,特别是TFA,并提高了USC-PFAS的灵敏度.

结论:

经过验证的HPLC-MS/MS方法提供了一个可靠的工具,用于在具有挑战性的食品矩阵中准确地确定USC-PFAS.
这些发现表明,番茄产品中可能存在内源性污染或与加工相关的PFBA积累.
这种分析进步有助于改善USC-PFAS的监管和食品安全评估.

关键词:

染色学西红超短链PFAS 食品安全问题

更多相关视频

Investigating Long-Distance Transport of Perfluoroalkyl Acids in Wheat via a Split-Root Exposure Technique

Investigating Long-Distance Transport of Perfluoroalkyl Acids in Wheat via a Split-Root Exposure Technique

Published on: September 28, 2022

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis

Published on: May 11, 2017

相关概念视频

Supercritical Fluid Chromatography

Supercritical Fluid Chromatography

Supercritical fluid chromatography (SFC) provides a beneficial substitute for gas chromatography (GC) and liquid chromatography (LC) for certain samples because it merges the top attributes of both techniques. SFC allows the separation and analysis of compounds that GC or LC does not easily manage. These compounds are traditionally nonvolatile or thermally unstable, making GC unsuitable and lacking functional groups required for HPLC analysis.
SFC utilizes a supercritical fluid mobile phase,...

Thin-Layer Chromatography (TLC): Overview

Thin-Layer Chromatography (TLC): Overview

Thin-layer chromatography (TLC) is a chromatography technique that separates compounds based on their polarity. TLC typically uses polar silica gel, a form of silicon dioxide, as the stationary phase. The silica gel contains hydroxyl (OH) groups on its surface, which form hydrogen bonds with polar compounds, influencing their adhesion to the stationary phase.
To begin the analysis, a mixture of compounds is spotted on the starting line on the TLC plate using a thin capillary. The bottom of the...

High-Performance Liquid Chromatography: Introduction

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process: