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阐明多二二氧化物与前列腺癌进展之间的相关性:基因表达和分子对接的见解
Fei Lin1, Yong Wei1, Chao-Ran Chen1
1Department of Urology, Urology Research Institute, the First Affiliated Hospital, Fujian Medical University, Fuzhou 350005, China; Department of Urology, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, Fuzhou 350212, China.
Ecotoxicology and environmental safety
|September 6, 2025
概括
多二二氧化物 (PCDD) 暴露与前列腺癌 (PCa) 风险和进展的增加有关. 这项研究开发了一种预后模型,并确定了机制,包括河马信号通路的改变,提供了对环境毒素对癌症的影响的见解.
科学领域:
- 环境健康科学
- 癌症学
- 毒理学
背景情况:
- 聚二二氧化物 (PCDD) 是具有已知的毒理性质的持久性环境污染物.
- 由于PCDD具有生物积累性,并带来长期的健康风险,因此需要对其在前列腺癌 (PCa) 等慢性疾病中的作用进行研究.
- 了解环境中毒物与癌症进展的分子机制对于公众健康至关重要.
研究的目的:
- 研究多二二氧化物 (PCDD) 暴露与前列腺癌 (PCa) 的发展和进展之间的相关性.
- 确定PCDD相关基因 (PRG) 并构建PCa的新预后模型.
- 阐明PCDD在PCa中的潜在生物机制,包括对细胞运动,增殖和信号通路的影响.
主要方法:
- 在线数据库 (CTD,TCGA,GEO) 的多维分析,以确定差异表达的PCDD相关基因 (PRG).
- 功能丰富分析 (GO,KEGG) 和预后模型的构建与内部/外部验证.
- 在体外 (EdU,Transwell,伤口愈合试验) 和体内 (裸体小鼠异种移植模型) 实验,分子对接和西部斑点/免疫光分析.
主要成果:
- 在与细胞运动,脂肪酸代谢和Hippo信号通路相关的途径中发现了217个差异表达的PRG.
- 一个与PCDD相关的新型预后模型准确地预测了多个群体的PCa无复发存活率.
- 八二二氧化物 (OCDD) 暴露在体内增强了PCa细胞的增殖,迁移,侵袭和瘤生长,同时抑制了Hippo信号通路.
结论:
- 暴露于PCDD与PCa进展的增加有关,这突显了环境毒素对癌症的影响.
- 基于PRG的验证预后模型可以帮助预测PCa患者的结果.
- 强迫症通过涉及Hippo信号通路的机制影响PCa的进展,这表明潜在的治疗点.
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