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Updated: Sep 8, 2025

Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation
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一种新型的动态流量平衡分析,用于模拟pH和温度变化的CHO细胞养批量培养

Ali Ghodba1, Anne Richelle2, Chris McCready3

  • 1Chemical Engineering Department, University of Waterloo, Waterloo, N2L 3G1, ON, Canada.

Journal of biotechnology
|September 6, 2025
PubMed
概括

这项研究引入了一种先进的动态流量平衡分析 (dFBA) 模型,用于预测中国仓鼠卵巢 (CHO) 细胞代谢. 在不同pH和温度条件下,优化模型准确预测细胞生长和单克隆抗体的产生.

关键词:
贝叶斯信息标准CHO 细胞动态流量平衡模型养批量培养其他pH和温度的变化

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Calculating pH Changes in a Buffer Solution02:45

Calculating pH Changes in a Buffer Solution

A buffer can prevent a sudden drop or increase in the pH of a solution after the addition of a strong acid or base up to its buffering capacity; however, such addition of a strong acid or base does result in the slight pH change of the solution. The small pH change can be calculated by determining the resulting change in the concentration of buffer components, i.e., a weak acid and its conjugate base or vice versa. The concentrations obtained using these stoichiometric calculations can be used...
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