通过向基因编辑,以β-胡卜素生物强化番茄
Xueting Liu1, Ying Fu1, Meng Li1
1Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Minhang, Shanghai, 200240, China.
International journal of biological macromolecules
|September 6, 2025
概括
这项研究使用CRISPR基因编辑增强了番茄中的β- 胡卜素. 生物丰富的西红增加了β-胡卜素,
科学领域:
- 植物生物技术
- 营养科学
- 农业科学
背景情况:
- 维生素A缺乏是一个全球性的健康危机.
- 番茄含有丰富的白素, 这使其适合生物强化.
- 在作物中增加β-胡卜素可以对抗微量营养素缺乏.
研究的目的:
- 通过CRISPR介导的基因编辑增加番茄中的β-胡卜素含量.
- 研究基因编辑对胡卜素生物合成途径的影响.
- 评估水果质量和收获后特征的潜在权衡.
主要方法:
- 使用CRISPR-Cas9技术产生了淘汰突变 (cr-SlLCYe和cr-SlBCH).
- 进行了胡卜素分析以量化β-胡卜素和烯水平.
- 对突变水果的特征进行全面评估,包括外观,营养质量和收获后的特征.
主要成果:
- 与野生番茄相比,突变番茄的β- 胡卜素含量高出1.7至2.5倍.
- 在产生的突变体中,甘含量保持不变.
- 没有观察到水果外观,营养质量或收获后特征的重大损害.
结论:
- 通过CRISPR进行基因编辑是增强番茄β-胡卜素的有效策略.
- 可以达到较高的β-胡卜素含量,而不会对水果的基本特性产生负面影响.
- 这项研究为开发生物丰富的番茄提供了一个有前途的方法来解决维生素A缺乏症.
更多相关视频
12:59High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots
Published on: April 30, 2016
18.1K
09:43Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
Published on: January 3, 2025
2.6K
相关概念视频
Transgenic Plants
7.4K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
7.4K
Plant Breeding and Biotechnology
19.7K
Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
19.7K
Transgenic Organisms
31.6K
Overview
31.6K
Gene Therapy
25.8K
Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be...
25.8K
What is Genetic Engineering?
75.4K
Overview
75.4K
