通过微生物链的延长,生物载体驱动的鱼产量增强:将代谢重定向与微生物组组合联系起来
Xinran Ji1, Kongyun Zhu2, Lei Zhang1
1Key Laboratory of Industrial Ecology and Environmental Engineering, Ministry of Education (MOE), School of Environmental Science and Technology, Dalian University of Technology, Linggong Road 2, Dalian 116024, China.
Bioresource technology
|September 6, 2025
概括
生物炭和纳米磁铁生物载体显著增加了从乙醇/乙酸生产的链延长. 这些材料增强了微生物群落和代谢途径,改善了中链脂肪酸产量.
科学领域:
- 微生物生态学
- 生物技术
- 环境工程
背景情况:
- 链延长 (CE) 是生产中链脂肪酸 (MCFA) 的关键生物过程.
- 优化CE效率和选择性仍然是一个挑战,常常受到产品抑制和微生物群体结构的限制.
- 生物载体通过提供支持和改变微观环境来增强微生物过程的潜力.
研究的目的:
- 评估五种不同的生物载体 (生物炭,活性炭,纳米磁铁,零价值铁,聚氨海绵) 对链延长性能的影响.
- 研究生物载体对微生物群体结构和代谢途径在CE期间的影响.
- 阐明生物载体增强MCFA生产的机制.
主要方法:
- 使用乙醇/乙酸盐作为基质进行了无氧批量实验.
- 五种生物载体对链延长效率和鱼产量的影响进行了测试.
- 进行了微生物群体分析 (16S rRNA基因测序) 和脂肪酸生物合成的基因丰度预测.
- 评估了产品的抑制和代谢转变.
主要成果:
- 所有测试的生物载体都在不同程度上增强了链条的延长.
- 与对照组相比,生物炭和纳米磁铁显著增加了酸盐产量,并减少了滞后阶段.
- 活性炭和生物炭在随后的循环中显著提高了对MCFA的碳选择性.
- 生物载体的添加导致了链延长合成的丰富,增加了微生物多样性,并提高了预测的脂肪酸生物合成基因丰度.
结论:
- 生物载体,尤其是生物炭和活性炭等含碳物质,有效增强链条延长和鱼产量.
- 生物载体减轻产品抑制,重组微生物协会,并重新编程代谢途径以改善MCFA的合成.
- 这些发现为在微生物组工程中利用特定的生物载体进行向的MCFA生产提供了机制的理解.
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