在三阴性乳腺癌中TROP2免疫组织化学的测试间变异性
Giulia Cursano1,2, Alberto Concardi1,2, Mariia Ivanova1
1Division of Pathology, IEO, European Institute of Oncology IRCCS, Via G. Ripamonti 435, 20141, Milan, Italy.
Molecular diagnosis & therapy
|September 6, 2025
概括
这项研究发现TROP2表达分析在三阴性乳腺癌的不同免疫组织化学测定中具有显著的变异性. 标准化这些测试对于可靠的TROP2生物标志物评估至关重要.
科学领域:
- 癌症学
- 免疫组织化学
- 生物标志物分析
背景情况:
- 在转移性三阴性乳腺癌 (TNBC) 中,sacituzumab govitecan可向TROP2.
- 通过免疫组织化学 (IHC) 评估的TROP2表达与改善的结果相关,但缺乏标准化测试.
- 这种变异性影响了TROP2作为生物标志物的可靠临床效用.
研究的目的:
- 在三种不同的IHC试验中评估TROP2表达分析的一致性.
- 使用H分数方法评估TROP2量化的一致性.
- 在TNBC TROP2生物标志物评估中确定标准化IHC协议的需要.
主要方法:
- 在Dako Omnis平台上使用三个不同的IHC测定 (ENZO-ABS380-0100,Abcam SP295,Santa Cruz B9-sc-376746) 分析了26个TNBC样本.
- 使用H分数量化TROP2膜表达,将结果分类为低,中等或高.
- 使用科恩的k和Gwet的AC2统计数据评估了测试一致性.
主要成果:
- 在三个试验中观察到TROP2表达分类的显著变化.
- 试验B和C显示了实质性的一致性 (80.8%的一致性, κ = 0.81),而所有三种试验的整体一致性均为相当或中等 (AC2 = 0.35).
- 与B和C试验相比,A试验显示了TROP2表达的范围更广.
结论:
- 在TROP2检测中存在显著的测试间差异,影响其作为TNBC生物标志物的可靠性.
- TROP2的表达是异质的和动态的,需要在IHC测试中改进方法.
- 需要标准化的IHC协议和定量评分系统,可能与计算病理学集成,以增强TROP2的临床效用.
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