重建转录的实时可视化揭示了单个促进者的RNAPII激活机制
Megan Palacio1, Dylan J Taatjes1
1Department of Biochemistry, University of Colorado, Boulder, CO 80303, USA.
Cell reports
|September 7, 2025
概括
转录因子和调解器中的内在失序区域 (IDR) 对于快速的RNA聚合酶II (RNAPII) 激活至关重要. 这项研究揭示了IDRs控制RNAPII功能和爆裂动力学,独立于相分离的凝聚物.
科学领域:
- 分子生物学分子生物学
- 生物化学 生物化学
- 遗传学 是一个遗传学.
背景情况:
- RNA聚合酶II (RNAPII) 转录是由转录因子 (TFs) 和前启动复合体 (PIC) 调节的.
- TFs,Mediator和RNAPII中的内在无序区域 (IDR) 与相分离有关,但它们在RNAPII功能中的确切作用尚不清楚.
研究的目的:
- 研究内在无序区域 (IDR) 在调节RNA聚合酶II (RNAPII) 活性和转录中的作用.
- 阐明TF和调解器控制RNAPII激活和破裂动态的机制.
主要方法:
- 实时体外光转录 (RIFT) 试验的开发,用于同时对数百个促进体的转录进行秒比秒的可视化.
- 利用纯化的PIC因子来研究体外转录动态.
主要成果:
- IDRs对于快速RNAPII激活至关重要,其功能独立于相隔凝结物形成.
- 调解者的IDR可以替代HSF1的功能,但表现出较慢的激活动力学.
- 调解器和TF通过他们的IDRs协同增强RNAPII爆发和重新启动.
- 在没有直接的TF-DNA结合的情况下,Mediator促进了TF-promoter的招聘.
结论:
- IDRs在RNAPII的快速激活中发挥着关键的,不依赖凝结物的作用.
- RIFT试验为转录爆发,IDR功能和增强剂-促进剂通信提供了新的机理洞察力,补充了活细胞研究.
关键词:
科普:分子生物学 分子生物学在HSF1中,它是HSF1.调解人 调解人 调解人在PIC的脚手架上.的RNA聚合酶II.五分之一 五分之一爆发大小 爆发大小 爆发大小爆发的爆发 爆发的爆发凝结剂是一种凝结剂.光显微镜的光显微镜.本质上是无序的地区.阶段分离的相位分离.重新启动重新启动.这就是SMTIRF.转录 转录 是一种转录.转录因子的转录因子更多相关视频
相关概念视频
RNA Polymerase II Accessory Proteins
10.8K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
10.8K
Co-activators and Co-repressors
8.4K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
8.4K
Eukaryotic Transcription Activators
12.5K
Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...
12.5K
Transcription Initiation
20.4K
Initiation is the first step of transcription in eukaryotes. Prokaryotic RNA Polymerase (RNAP) can bind to the template DNA and start transcribing. On the other hand, transcription in eukaryotes requires additional proteins, called transcription factors, to first bind to the promoter region in the DNA template. This binding helps recruit the specific RNAP that can assemble on the DNA and start transcription.
The promoters and enhancers and their accessory proteins allow tight regulation of...
The promoters and enhancers and their accessory proteins allow tight regulation of...
20.4K
Prokaryotic Transcriptional Activators and Repressors
10.3K
10.3K
Prokaryotic Transcriptional Activators and Repressors
25.1K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...
25.1K


