多重聚合酶连锁反应和复杂食品矩阵的测试:对大肠杆菌和沙门氏菌spp的初步研究
Stéphanie Watier-Grillot1, Christian Sauvageot2, Evelyne Thiébaut2
1French Armed Forces Centre for Epidemiology and Public Health (CESPA), Marseille, France.
Journal of microbiological methods
|September 7, 2025
概括
在食品传播疾病爆发调查中,BioFire® FilmArray®胃肠道面板 (BF-GIP) 对检测沙门氏菌和大肠杆菌等食品传播病原体充满希望. 然而,由于特异性问题,大肠杆菌的鉴定需要用传统方法进行确认.
科学领域:
- 食品微生物学 食品微生物学
- 分子诊断学 分子诊断学
- 食品传播疾病监测 食品传播疾病监测
背景情况:
- 食物传播疾病表现出类似的消化系统症状,因此在疫情调查中需要采用综合征方法.
- 多重PCR方法对于在疫情期间分析便和食品样本至关重要.
研究的目的:
- 评估BioFire® FilmArray®胃肠道面板 (BF-GIP) 作为一种替代食品微生物学方法.
- 根据ISO 16140-2标准评估BF-GIP的性能,以检测大肠杆菌和沙门氏菌spp. 在混合沙拉中.
主要方法:
- 对BF-GIP与基于文化的参考方法进行评估.
- 测试了205个自然和人工污染的混合沙拉样本.
- 对大肠杆菌和沙门氏菌的分析. 检测. 检测. 在检测.
主要成果:
- 据BF-GIP证明,对沙门氏菌的敏感度为100%. 有2.4%的错误阳性.
- BF-GIP对大肠杆菌的敏感度为92.9%,与参考方法相比.
- 包括性和排他性研究显示,人工大肠杆菌菌株的错误率分别为14%和9%.
结论:
- BF-GIP是一种可行的替代方案,用于查沙门氏菌spp. 在疫情调查期间,食品矩阵中的大肠杆菌和大肠杆菌.
- 由于潜在的特异性问题,建议使用参考方法确认大肠杆菌菌株.
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