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一个SERS活性微针阵列,用于快速和最少侵入性的乳酸检测

Xinyu Sun¹, Kequan Chen², Yang Wang¹

¹State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China.

Analytica chimica acta

|September 7, 2025

概括

这项研究引入了一种新的微针阵列,用于检测间歇性液体中的乳酸 (LA),为监测运动强度和运动员表现提供了一种更安全的方法. 调查结果显示,禁食和食状态之间的LA产量存在差异,影响了培训指导.

科学领域:

生物医学工程生物医学工程
运动科学运动科学运动科学
分析化学分析化学

背景情况:

剧烈的运动会导致乳酸 (LA) 积累,导致肌肉疲劳并影响生理功能.
乳酸值 (LT) 对运动表现至关重要,使LA检测对评估身体状况和优化训练至关重要.
目前用于LA检测的方法可能是侵入性的或缺乏灵敏度,需要先进的监测工具.

研究的目的:

开发和验证表面增强的拉曼散射 (SERS) 活性微针 (MN) 阵列,用于实时检测间歇性流体中的LA.
研究生理状态 (禁食与正常食) 和运动强度对LA积累的影响.
评估SERS-MN阵列在指导运动训练和康复方面的潜力.

主要方法:

通过整合黄金纳米,4-mercaptobenzeneboronic acid和乳酸氧化酶来制造SERS活性微针阵列.
将MN阵列插入小鼠皮肤,以检测间歇性液体 (ISF) 中的LA.
监测不同运动持续时间和食条件下的小鼠ISF LA度的变化.

主要成果:

在SERS-MN阵列成功地检测到LA度在老鼠ISF运动后LA度的变化.
在正常小鼠中,游泳3分钟和6分钟后,ISF LA显著增加.
与正常食小鼠相比,禁食小鼠在运动期间表现出改变的基线LA水平和不同的积累率.

结论:

这项研究首次确定了禁食和正常养的动物之间明显的LA生产模式.
禁食影响了基线LA水平和运动诱导的LA积累率.
开发的SERS-MN阵列显示了作为一种更安全,更有效的炼监测和个性化训练指导工具的承诺.

关键词:

间歇性流体间歇性流体乳酸检测乳酸检测乳酸检测微针阵列是一个微针阵列.在表面增强的拉曼散射中.

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