一种系统应用的基于纳米粒子的dsRNA生物杀虫剂降低了Tuta absoluta的生存率
William T Askew1, Angharad M R Gatehouse2, Martin G Edwards2
1Newcastle University, School of Natural and Environmental Sciences, Newcastle upon Tyne, UK NE1 7RU; Natural England, 4(th) Floor, Kings Pool, 1-2 Peasholme Green, York YO1 7PX, UK.
Pesticide biochemistry and physiology
|September 7, 2025
概括
RNA干扰 (RNAi) 通过向瑞亚诺丁受体 (RyRs) 来有效控制番茄害虫Tuta absoluta. 基托桑纳米配制的dsRNA提供了稳定,安全和有效的生物杀虫剂解决方案,用于作物保护.
科学领域:
- 农业昆虫学 农业昆虫学
- 分子生物学分子生物学
- 生物技术是生物技术.
背景情况:
- RNA干扰 (RNAi) 是一种用于害虫控制的基因沉默机制.
- 一种主要的番茄害虫Tuta absoluta由于RyR突变而对二氧化杀虫剂表现出耐药性.
- 开发基于RNAi的生物农药对于可持续农业至关重要.
研究的目的:
- 为了评估RNA干扰向Ryanodine受体 (RyRs) 在 Tuta absoluta中的有效性.
- 评估一种色素纳米配方的dSRNA的稳定性和安全性,其向的是TaRy.
- 为T. absoluta.提供一个环境安全的RNAi控制策略.
主要方法:
- 在色素纳米配方中对Tuta absoluta RyR特异性 (TaRy) dsRNA的外源性应用,用于水培栽培的番茄植物.
- 监测基因表达,昆虫死亡率和叶子损伤.
- 在非目标关节动物的体外生物安全评估.
主要成果:
- 在T. absoluta. 中,TaRy dsRNA显著降低了RyR基因表达的调节.
- dsRNA治疗导致了大量的昆虫死亡,减少了作物损害.
- 素纳米配方增强了植物和昆虫组织中的TaRy dsRNA稳定性.
结论:
- 通过奇多纳米配方输送的TaRy dsRNA是一种有效和稳定的抗T. absoluta.生物杀虫剂.
- 这种方法表明,在最小的环境风险下,有显著的害虫控制.
- 这项研究提出了一个有希望的基于RNAi的战略,用于在农业中管理T. absoluta.
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