衰老调节剂重塑介质干细胞-施万细胞电路,用于糖尿病患者的骨再生
An Lao1, Yiting Sun2, Weiqi Li3
1Department of Stomatology, Xin Hua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China; Department of Oral and Cranio-maxillofacial Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, 200011, China.
Biomaterials
|September 8, 2025
概括
糖尿病骨缺陷通过影响施万细胞 (SCs) 损害愈合. 一种新的纳米粒子系统 (MMZ@Si-P53/HAMA-PBA-PVA) 能够使衰老的SCs复苏,恢复细胞电路,并促进糖尿病大鼠的骨再生.
科学领域:
- 生物材料科学 生物材料科学
- 再生医学是一种再生医学.
- 糖尿病并发症 糖尿病并发症
背景情况:
- 骨愈合依赖于施万细胞 (SC) 的膜信号传递,用于介质干细胞 (MSC) 的功能.
- 糖尿病 (DM) 损害了SC功能,导致骨再生有缺陷.
- 在糖尿病骨修复中重建MSC-SC电路仍未得到充分探索.
研究的目的:
- 开发一个有针对性的纳米粒子系统,用于在糖尿病微环境中使衰老的SCs复苏.
- 研究该系统在增强糖尿病患者骨再生方面的治疗潜力.
主要方法:
- 将针对P53的小干扰RNA (Si-P53) 和甲胺 (MET) 联合封装成ZIF-8纳米粒子 (MMZ@Si-P53).
- 将MMZ@Si-P53装入一种对葡萄糖有反应的水凝 (MMZ@Si-P53/HAMA-PBA-PVA) 中,以进行有针对性的输送.
- 评估SC衰老,线粒体功能,血清蛋白生物合成,MSC衰老和骨化.
- 在老鼠模型中评估糖尿病骨缺陷修复.
主要成果:
- 糖尿病SCs表现出衰老,过度反应性氧物种 (ROS) 和线粒体功能障碍.
- MMZ@Si-P53治疗促进了SC线粒体的恢复和血清蛋白生物合成,使SCs复苏.
- 复原的SC恢复了MSC-SC电路,抑制了MSC衰老并增强了骨化.
- MMZ@Si-P53/HAMA-PBA-PVA水凝系统在体内有效促进了糖尿病患者的骨再生.
结论:
- 开发的纳米粒子-水凝系统有效地准并使糖尿病中老化的SCs复苏.
- 这种方法恢复了关键的MSC-SC交叉,这对骨再生至关重要.
- 这项研究为治疗糖尿病骨缺陷提供了一个有前途的治疗策略.
相关概念视频
01:19Mesenchymal Stem Cells
5.5K
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
5.5K
01:22iPS Cell Differentiation
3.0K
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
3.0K
01:23Tissue Renewal without Stem Cells
2.1K
After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
However, failure of such a system...
However, failure of such a system...
2.1K
01:17Stem Cell Culture
6.0K
Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
6.0K
01:26Stem Cell Niche
6.2K
The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
6.2K
01:15Neurogenesis and Regeneration of Nervous Tissue
1.6K
In the CNS, neurogenesis, the birth of new neurons from stem cells, is limited to the hippocampus in adults. In other regions of the brain and spinal cord, neurogenesis is almost non-existent due to inhibitory influences from neuroglia, especially oligodendrocytes, and the absence of growth-stimulating cues. The myelin produced by oligodendrocytes in the CNS inhibits neuronal regeneration. Furthermore, astrocytes proliferate rapidly after neuronal damage, forming scar tissue that physically...
1.6K