固体器官移植中来自捐赠者的无细胞DNA:分析考虑,诊断性能和临床解释
Hyunhye Kang1,2, Sang-Ik Cho3, Eun-Jee Oh4,5
1Department of Laboratory Medicine, College of Medicine, Graduate School, The Catholic University of Korea, Seoul, Korea.
Clinical transplantation and research
|September 8, 2025
概括
来自捐赠者的无细胞DNA (dd-cfDNA) 是固体器官移植损伤的有希望的非侵入性生物标志物. 虽然它有助于排除排斥,但它的解释需要考虑临床背景,因为潜在的非特异性升高.
科学领域:
- 移植免疫学 移植免疫学
- 分子诊断学 分子诊断学
- 发现生物标志物的发现.
背景情况:
- 捐赠者衍生的无细胞DNA (dd-cfDNA) 来自受伤的移植器官.
- 基线dd-cfDNA水平受到器官类型和移植后的生理变化的影响.
研究的目的:
- 审查dd-cfDNA作为非侵入性生物标志物用于全移植损伤的实用性.
- 讨论分析平台,解释挑战和dd-cfDNA的未来方向.
主要方法:
- 对固体器官移植中的dd-cfDNA现有文献的综述.
- 分析各种分析平台 (qPCR,ddPCR,NGS) 和量化方法.
- 讨论影响dd-cfDNA水平和诊断值的因素.
主要成果:
- dd-cfDNA是一种有价值的生物标志物,具有高负预测值,可能减少活检.
- 商业测定在多个器官中得到验证.
- dd-cfDNA不是针对排斥的特异性,并且可以通过感染,缺血或炎症升高.
结论:
- dd-cfDNA在非侵入性监测全移植损伤方面显示出显著的前景.
- 考虑到生物变异,试验特点和临床背景的仔细解释至关重要.
- 为了更广泛的采用,需要进一步的标准化,器官特定的门和成本效益研究.
更多相关视频
11:59Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies
Published on: September 6, 2017
7.8K
05:26A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications
Published on: September 16, 2022
4.6K
相关概念视频
Kidney Transplant I: Introduction
349
A kidney transplant is a surgical approach that involves replacing a non-functioning kidney with a healthy one from a donor. This procedure is often a treatment option for end-stage renal disease (ESRD) patients. The method requires careful recipient selection, including evaluating various medical and psychosocial factors. These criteria vary between transplant centers but generally include assessments of the patient's overall health, adherence to medical recommendations, and lifestyle...
349
Bone Marrow Sampling and Transplants
910
Bone marrow transplant is a potential cure for several diseases, including cancer and specific genetic disorders. Notably, this procedure is applicable for patients suffering from aplastic anemia, certain types of leukemia, severe combined immunodeficiency disease (SCID), Hodgkin's disease, non-Hodgkin's lymphoma, multiple myeloma, thalassemia, sickle-cell disease, and certain cancers.
The transplant begins with high doses of chemotherapy and radiation treatment, which aim to destroy...
The transplant begins with high doses of chemotherapy and radiation treatment, which aim to destroy...
910
Tissue Transplantation
928
Tissue transplantation is a significant medical procedure involving the transfer of cells, tissues, or organs from a donor to a recipient, with the primary aim of restoring lost functions. This procedure is crucial in treating a broad spectrum of diseases, including kidney diseases, liver failure, heart disease, and certain types of cancers.
The Biology of Tissue Transplantation
The biology of tissue transplantation hinges on the Major Histocompatibility Complex (MHC) molecules. These molecules...
The Biology of Tissue Transplantation
The biology of tissue transplantation hinges on the Major Histocompatibility Complex (MHC) molecules. These molecules...
928
