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相关实验视频

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扩大加拿大北极地区潜在入侵物种的监测能力,使用环境DNA元编码.

Elizabeth Boyse1, Melody S Clark1, Ian M Carr2

  • 1British Antarctic Survey, Cambridge, UK.

Global change biology
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概括

环境DNA (eDNA) 调查在加拿大北极地区发现了入侵性海湾,这凸显了随着气候变化和海运增加,对非土著物种造成新的风险,需要进行监测.

关键词:
在北极,北极的北极.气候变化 气候变化 气候变化环境 DNA DNA 环境 DNA人类对人类的影响.侵入性物种是一种入侵性物种.超级编码元标段编码.监控 监控 监控 监控 监控 监控运输运输运输的交通.

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科学领域:

  • 海洋生物学 海洋生物学
  • 环境科学环境科学
  • 基因组学就是基因组学.

背景情况:

  • 北极加拿大历史上孤立的环境限制了非土著物种的建立.
  • 气候变化和海运流量的增加 (自1990年以来超过250%) 正在促进物种引入.
  • 不完整的北极生物库存阻碍了新或入侵物种的识别.

研究的目的:

  • 通过使用环境DNA (eDNA) 的metabarcoding来评估真核生物群落的组成,并检测加拿大北极地区潜在的入侵物种.
  • 调查西北通道沿线的生物多样性,这是一个主要的航道,目标是乘客船经常通行的地区.
  • 验证eDNA元编码作为数据贫困地区生物多样性监测的工具.

主要方法:

  • 从西北通道的27个地点收集了1升的海水样本.
  • 利用了环境DNA (eDNA) 的metabarcoding,针对18SrRNA V9和COI区域.
  • 分析了真核生物群体的组成,并确定了Amplicon序列变异 (ASV).

主要成果:

  • 检测到126个COI ASV和391个18S ASV,提供了真核生物社区组成的快照.
  • 确认了copepods,dinoflagellates和 diatoms作为主导群体,与之前的净采样调查保持一致.
  • 报道了加拿大北极地区首次发现入侵性海湾 (Amphibalanus improvisus).

结论:

  • 环境DNA (eDNA) 是有效的检测非土著物种在不足样本的北极地区.
  • 检测到Amphibalanus improvisus标志着潜在的新入侵,需要进一步调查其繁殖状况.
  • 将eDNA与公民科学和当地知识相结合,可以在不断变化的环境中为北极入侵物创造一个强大的监测系统.