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相关概念视频

CRISPR01:59

CRISPR

57.5K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
57.5K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

1.7K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.7K
Homologous Recombination02:31

Homologous Recombination

62.7K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
62.7K
What is Genetic Engineering?00:49

What is Genetic Engineering?

79.7K
Overview
79.7K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.7K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.7K
CRISPR and crRNAs02:53

CRISPR and crRNAs

18.7K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
18.7K

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相关实验视频

Updated: Jan 18, 2026

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
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Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

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与CRISPR回归基因驱动器相关的风险

Virginie Courtier-Orgogozo

    Comptes rendus biologies
    |September 8, 2025
    PubMed
    概括
    此摘要是机器生成的。

    克里斯普尔指向基因驱动器提供潜在的好处,但带有重大风险. 本审查详细介绍了技术限制和不良影响,包括生态和社会学方面的问题,以指导负责任的决策.

    关键词:
    生物安全 生物安全生物安全是生物安全.生物技术是生物技术.生物武器是一种生物武器.克里斯普尔是什么意思?克里斯普尔是什么意思?基因驱动器是基因驱动器.风险 风险 风险 风险 风险

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    Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology
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    Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology

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    Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
    11:35

    Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

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    相关实验视频

    Last Updated: Jan 18, 2026

    Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
    08:32

    Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

    Published on: May 23, 2025

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    Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology
    10:07

    Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology

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    Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
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    Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

    Published on: June 16, 2017

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    科学领域:

    • 生物技术是生物技术.
    • 基因工程是一种基因工程.
    • 保护生物学 保护生物学

    背景情况:

    • 克里斯普尔定向基因驱动是一种新的生物技术,在公共卫生,农业和自然保护方面具有潜在的应用.
    • 基因驱动是自主自私的遗传元素,旨在通过自然种群传播.
    • 尽管有潜力,但CRISPR指向基因驱动器尚未在野生环境中部署.

    研究的目的:

    • 检查与CRISPR回归基因驱动器相关的关键风险.
    • 探索基因驱动系统的技术局限性和潜在故障.
    • 分类和讨论不良影响,包括生态,社会学,研究相关的和恶意使用风险.

    主要方法:

    • 对关于CRISPR指向基因驱动器的现有文献和研究进行审查.
    • 分析技术局限性,包括效率和缓解策略的失败.
    • 将潜在的不利影响分类为生态,社会学,研究和恶意使用.

    主要成果:

    • 基因驱动可能面临技术限制,影响其预期的效率和缓解策略的有效性.
    • 生态风险包括对生态系统和非目标种群的意外后果.
    • 社会风险包括公众的看法,治理挑战和社会接受问题.
    • 风险也扩展到研究活动和潜在的恶意应用.

    结论:

    • 对CRISPR定位基因驱动风险的彻底评估对于负责任的开发和部署至关重要.
    • 了解技术限制和各种不利影响对于知情决策至关重要.
    • 这一审查为评估基因驱动风险提供了基础,以确保安全和道德的进步.