在小鼠的正常受精和人工卵细胞激活中,需要溶解体双孔通道
Tadashi Yamazaki1, Md Wasim Bari2, Satoshi Kishigami1,2,3,4
1Department of Integrated Applied Life Science, Integrated Graduate School of Medicine, Engineering, and Agricultural Sciences, University of Yamanashi, 400-8510 Yamanashi, Japan.
Frontiers in bioscience (Landmark edition)
|September 8, 2025
概括
溶解体通道 (TPC) 对于小鼠卵细胞激活和受精至关重要. 用trans-Ned19抑制TPC破坏了这些过程,并损害了精子功能,突出了它们的重要作用.
科学领域:
- 生殖生物学 生殖生物学
- 细胞生物学 细胞生物学
- 信号传递 信号传递
背景情况:
- 溶解体在细胞降解中起作用,并作为细胞内 (Ca2+) 储存器.
- 双孔通道 (TPC) 是溶解体通道,涉及各种细胞过程.
研究的目的:
- 通过使用TPC抑制剂trans-Ned19.9研究 lysosomal TPCs在小鼠受精和卵细胞激活中的作用.
主要方法:
- 评估了前核形成 (Hoechst 33342染色).
- 评估皮质颗粒的释放 (LCA-FITC染色).
- 监测细胞内的Ca2+水平 (Cal-520 AM).
- 分析精子运动率 (SMAS).
主要成果:
- 跨Ned19抑制了Sr2+诱导的卵细胞激活,包括前核形成,皮质颗粒释放和Ca2+振荡.
- 在试管婴儿中,trans-Ned19降低了受精率和降低了精子运动.
- 没有透视区的卵子细胞受精率呈现出类似的情况,但在trans-Ned19时观察到多精子症的增加.
结论:
- 溶解体TPC对小鼠正常受精和人工卵细胞激活至关重要.
- 准TPC影响关键的生殖事件和精子功能.
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