在小鼠胎儿发育期间,昼夜钟基因表达的本体发生
Daniel L Stanton1,2, Linkai Zhu1, Peter J Hansen1
1Department of Animal Sciences, D.H. Barron Reproductive and Perinatal Biology Research Program, and Genetics Institute, University of Florida, Gainesville, FL, USA.
Biochemistry and biophysics reports
|September 8, 2025
概括
这项研究表明,核心昼夜钟基因表达在胎儿小鼠组织中以不同的速度成熟. 与心脏和脏相比,大脑和肝脏表现出较早的分子时钟组织,在出生后继续发育.
科学领域:
- 时间生物学 时间生物学
- 发展生物学 发展生物学
- 分子遗传学 分子遗传学
背景情况:
- 昼夜时钟在24小时周期内调节生理和细胞系统.
- 哺乳动物昼夜钟机制的发展时间表仍然不完全理解.
研究的目的:
- 研究哺乳动物发育过程中昼夜钟基因表达的本体发生.
- 确定核心昼夜钟基因转录在胎儿和产后小鼠组织中的时间模式.
主要方法:
- 利用以前发表的来自小鼠的RNA测序数据集.
- 分析了六个核心昼夜钟基因 (Clock,Bmal1,Per1,Per2,Cry1,Cry2) 的转录丰度.
- 检查了胎儿和产后阶段的大脑,心脏,肝脏和脏组织中的基因表达.
主要成果:
- 所有六个核心昼夜钟基因在所有检查的组织和发育时间点中都被检测到.
- 大多数核心时钟基因的转录丰度在妊娠晚期增加,特别是在大脑和肝脏.
- 基因表达模式表明,与胎儿心脏和脏相比,胎儿大脑和肝脏的分子时钟组织更先进,在出生后继续成熟.
结论:
- 分子时钟的组织在胎儿组织中以不同的方式建立,大脑和肝脏显示较早的发育.
- 循环时钟基因表达在出生后继续成熟,这表明长期的发育过程.
- 这些发现有助于更完整地了解哺乳动物的昼夜钟本体发生.
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