Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

DNA Replication02:40

DNA Replication

58.6K
DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
58.6K
The DNA Replication Fork01:02

The DNA Replication Fork

40.5K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
40.5K
The DNA Replication Fork01:02

The DNA Replication Fork

18.2K
18.2K
Replication in Eukaryotes01:29

Replication in Eukaryotes

17.1K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
17.1K
Replication in Eukaryotes02:31

Replication in Eukaryotes

203.5K
Overview
203.5K
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

112.7K
Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
112.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Reflections on emerging therapies for inherited retinal diseases following a community education event: A qualitative exploration.

Optometry and vision science : official publication of the American Academy of Optometry·2026
Same author

Global high-resolution mapping of seagrass to support conservation.

Nature·2026
Same author

Unexpected expansion and regrowth in Earth's mangrove forests over the past four decades.

Science (New York, N.Y.)·2026
Same author

The accelerating loss and shifting dynamics of US tidal wetlands.

Nature communications·2026
Same author

Retinoic acid drives cell fate specification, maturation and retinal regionality in human retinal organoids.

Nature communications·2026
Same author

Transduction of AAV Vectors in iPSC-Derived Retinal Organoids.

Methods in molecular biology (Clifton, N.J.)·2026

相关实验视频

Updated: Jan 18, 2026

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
08:30

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Published on: January 7, 2020

13.9K

开放EMMU:一个多功能,开源的EDU复杂化方法,用于研究DNA复制和细胞周期动态.

Osvaldo Contreras1,2, Chris Thekkedam1, John Zaunders3

  • 1Victor Chang Cardiac Research Institute, Darlinghurst, NSW 2010, Australia.

iScience
|September 8, 2025
PubMed
概括

开放EMMU是一种新的,负担得起的,开源的方法,用于跟踪使用5-Ethynyl-2'-deoxyuridine (EdU) 点击化学的DNA复制. 它提高了多种细胞类型和生物标本的染色效率和多重化.

关键词:
生物化学 生物化学细胞生物学 细胞生物学计算生物信息学是指计算机生物信息学.发育生物学是发展生物学.

更多相关视频

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
17:03

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay

Published on: March 23, 2010

19.3K
Author Spotlight: Unraveling the Dynamics of Eukaryotic DNA Replication Through Single-Molecule Visualization
07:37

Author Spotlight: Unraveling the Dynamics of Eukaryotic DNA Replication Through Single-Molecule Visualization

Published on: September 27, 2024

2.4K

相关实验视频

Last Updated: Jan 18, 2026

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
08:30

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Published on: January 7, 2020

13.9K
Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
17:03

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay

Published on: March 23, 2010

19.3K
Author Spotlight: Unraveling the Dynamics of Eukaryotic DNA Replication Through Single-Molecule Visualization
07:37

Author Spotlight: Unraveling the Dynamics of Eukaryotic DNA Replication Through Single-Molecule Visualization

Published on: September 27, 2024

2.4K

科学领域:

  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学
  • 发展生物学 发展生物学

背景情况:

  • 通过点击化学,5-乙烯基-2'-脱氧氨 (EdU) 对于DNA复制和细胞周期分析至关重要.
  • 商业EdU套件昂贵,专有,并且具有有限的多重复和样本兼容性.

研究的目的:

  • 开发一个优化,负担得起,和用户友好的EdU点击化学平台.
  • 为了提高EDU染色效率,亮度和多重复合功能.
  • 为了在各种生物环境中实现DNA合成的高分辨率成像.

主要方法:

  • 开发了OpenEMMU,这是一个开源平台,用于用于EDU检测的现成试剂.
  • 使用非结合和结合抗体进行多重染色.
  • 将该方法应用于各种细胞类型,包括T细胞,胚胎组织,有机体和斑马鱼幼虫.

主要成果:

  • OpenEMMU 证明了 EdU 染色的增强效率,亮度和复杂化.
  • 在发育中的胚胎,器官 (心脏,前肢) 和心脏器官中成功成像新生的DNA合成.
  • 启用了斑马鱼幼虫中DNA合成的深层组织3D成像,并在高空间分辨率的复制压力下进行复制.

结论:

  • OpenEMMU为研究DNA复制动态提供了一个灵活而精确的平台.
  • 这种方法促进了生物发育,细胞增殖和DNA合成方面的研究.
  • 为商业 EdU 套件提供了一个具有成本效益和多功能性的替代方案.