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相关概念视频

Applications Of NMR In Biology01:25

Applications Of NMR In Biology

4.4K
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
4.4K
RNA Structure01:23

RNA Structure

78.8K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
78.8K
RNA Structure01:19

RNA Structure

7.2K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
7.2K
RNA Stability01:53

RNA Stability

35.6K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
35.6K
Ribosome Profiling02:24

Ribosome Profiling

4.1K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.1K
RNA-seq03:21

RNA-seq

11.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.8K

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相关实验视频

Updated: Jan 18, 2026

Practical Aspects of Sample Preparation and Setup of 1H R1&#961; Relaxation Dispersion Experiments of RNA
08:17

Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA

Published on: July 9, 2021

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对于研究RNA结构和动态的固态NMR方法的进展.

Jinhan He1, Xiaole Liu1, Shenlin Wang1

  • 1State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.

Magnetic resonance letters
|September 8, 2025
PubMed
概括
此摘要是机器生成的。

固态核磁共振 (SSNMR) 为研究核酸 (RNA) 结构和动态提供了一种强大的方法. 本综述强调了用于RNA研究的1H检测SSNMR技术的进步.

关键词:
动力学 动力学 动力学脉冲序列的脉冲序列是什么这是一个RNARNARNARNARNA.固态NMR是一种固态NMR.结构 结构 是一个结构.

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Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
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Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

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相关实验视频

Last Updated: Jan 18, 2026

Practical Aspects of Sample Preparation and Setup of 1H R1&#961; Relaxation Dispersion Experiments of RNA
08:17

Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA

Published on: July 9, 2021

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Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
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Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

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科学领域:

  • 生物物理学的生物物理.
  • 结构生物学 结构生物学
  • 生物化学 生物化学

背景情况:

  • 核糖核酸 (RNA) 的结构和动态对于理解生物功能和设计针对RNA的药物至关重要.
  • 像X射线结晶学和溶液核磁共振 (NMR) 这样的传统方法面临着RNA灵活性和降解的挑战.
  • 固态NMR (SSNMR) 已经成为RNA结构和动态表征的强大替代方案.

研究的目的:

  • 为RNA结构和动态的1H检测SSNMR提供最近进展的全面审查.
  • 讨论已建立的1H检测SSNMR方法和样本准备协议.
  • 突出RNA SSNMR中快速数据采集方法的实施.

主要方法:

  • 专注于1H检测的固态核磁共振 (SSNMR) 技术.
  • 详细讨论RNA的样本准备协议.
  • 探索快速数据采集策略以提高效率.

主要成果:

  • 1H检测的SSNMR方法已经进一步阐明了固态RNA拓和基对动态.
  • 应用包括确定人类免疫缺陷病毒 (HIV) 基因组中的RNA结构和核糖核酸切换RNA.
  • SSNMR技术证明了适用于各种RNA大小和样本状态的适用性.

结论:

  • 固态NMR是RNA结构和动态研究的有价值和多功能工具.
  • 1H检测SSNMR的进步显著扩大了其在RNA领域的实用性.
  • 本综述巩固了关键的方法和发现,为未来的RNA结构研究铺平了道路.