相关实验视频

Updated: Jan 18, 2026

Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells

Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells

Published on: July 12, 2022

一个序列激活的近红外光探测器,用于精确跟踪in vivo衰老

Jiahuan Nong¹, Pengcheng Li¹, Mingming Zhu²

¹Shanghai Key Laboratory of Functional Materials Chemistry, Institute of Fine Chemicals, Frontiers Science Center for Materiobiology and Dynamic Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology Shanghai 200237 China guozq@ecust.edu.cn.

Chemical science

|September 8, 2025

概括

精确检测衰老细胞对于衰老研究至关重要. 一种新的双模式近红外 (NIR) 探测器,MFB-βgal,通过检测β-银酸酶活性和溶酶化,精确地识别衰老细胞,改善细胞衰老监测.

科学领域:

生物医学工程生物医学工程
细胞生物学细胞生物学
老年学是一门学科.

背景情况:

实时监测衰老细胞对于衰老研究和干预至关重要.
现有的探针仅依赖于β-galactosidase (β-gal) 活性,由于非特异性酶表达,可能导致不准确的衰老细胞检测.
开发精确的老化细胞识别方法对于理解衰老过程至关重要.

研究的目的:

设计和验证一种新的双模式顺序响应和逻辑近红外 (NIR) 探测器,用于准确检测衰老细胞.
为了克服仅依赖于β-galactosidase活性的探针的局限性.
为实时可视化和监测细胞衰老提供一种新的策略.

主要方法:

设计了一种双模式序列响应和逻辑NIR探测器 (MFB-βgal),其中包含一个β-gal可切割单元和一个形态素部分.
在与β-gal反应时,MFB-βgal在现场产生MFB-OH,从而能够检测出溶解体化.
利用探头在SKOV-3和衰老细胞之间进行区分,并在小鼠模型中进行体内可视化.

主要成果:

MFB-βgal 探针在 β-gal 酶活性后成功地在现场生成了 MFB-OH.
探测器准确地检测到溶酶体化,这是衰老细胞的特征.
在小鼠模型中成功区分了SKOV-3和衰老细胞,并可视化了β-gal活动in vivo.

结论:

开发的MFB-βgal探头为实时衰老细胞检测提供了一个新的,准确的策略.
双模式和逻辑方法通过结合酶活性和 lysosomal 向来增强特异性.
这种探测器有助于更好地了解和监测细胞衰老和衰老过程.

更多相关视频

In Vivo Quantification of Protein Turnover in Aging C. Elegans using Photoconvertible Dendra2

In Vivo Quantification of Protein Turnover in Aging C. Elegans using Photoconvertible Dendra2

Published on: June 13, 2020

Far-Red Fluorescent Senescence-Associated β-Galactosidase Probe for Identification and Enrichment of Senescent Tumor Cells by Flow Cytometry

Far-Red Fluorescent Senescence-Associated β-Galactosidase Probe for Identification and Enrichment of Senescent Tumor Cells by Flow Cytometry

Published on: September 13, 2022

相关概念视频

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...