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肝中主要细菌病原体的量化和牛和结肠上皮组织匹配的牛和结肠上皮组织

Mina Abbasi¹, Alyssa Deters¹, Harith Salih²

¹Department of Diagnostic Medicine and Pathobiology, Kansas State University, , Manhattan, Kansas, USA.

Microbiology spectrum

|September 8, 2025

概括

一种新的qPCR试验准确量化了牛肝 (LA) 中的细菌病原体. Fusobacterium necrophorum是占主导地位的物种,而Trueperella pyogenes和Salmonella enterica对LA发展的贡献有限.

科学领域:

兽医微生物学兽医微生物学
牛病理学牛病理学
分子诊断学分子诊断

背景情况:

牛的肝 (LA) 是一个重大的经济挑战,源于多微生物感染.
涉及的关键细菌病原体包括Fusobacterium necrophorum (FNN, FNF),Trueperella pyogenes (TP) 和沙门氏菌 (SE).
评估单个物种在多微生物感染中的贡献是具有挑战性的.

研究的目的:

开发和验证一个四复数定量PCR (qPCR) 试验,用于精确检测和量化牛中主要的细菌病原体.
确定LA中的FNN,FNF,TP和SE的患病率和度,以及匹配的反 (RT) 和结肠组织 (CT).

主要方法:

开发一种针对FNN,FNF,TP和SE的4个复数qPCR试验.
分析了384个LA样本和匹配的RT (374) 和CT (256) 组织.
将qPCR结果与传统基于培养的方法进行比较.

主要成果:

在LA地区,Fusobacterium necrophorum (FNN/FNF) 的流行率很高 (85.9%),度很高 (7-7.5 log10 CFU/g).
在29%的LA中检测到Trueperella pyogenes,但度较低 (5.9 log10 CFU/g),这表明它起到了次要的作用.
沙门氏菌在LA中无法通过qPCR量化,这表明沙门氏菌对瘤形成的贡献很小.

结论:

开发的qPCR测定是一种可靠的工具,用于量化牛LA的关键病原体.
Fusobacterium necrophorum 是牛中LA的主要驱动因素.
真菌和沙门氏菌似乎在LA的发病过程中起到轻微或无助于发病的作用.

关键词:

这种细菌名为Fusobacterium necrophorum.沙门氏菌 (Salmonella enterica) 已经成为一种常见的疾病.真贝拉 (Trueperella pyogenes) 是一种天然的植物.肝脏肝脏 qPCRR 是一个很好的方法.排骨和结肠上皮组织.

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