含有三种二硫化物的S-糖化糖的表征
Rachel M Martini1, Chandrashekhar Padhi2, Wilfred A van der Donk1,2,3
1Department of Biochemistry University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Journal of industrial microbiology & biotechnology
|September 8, 2025
概括
在大肠杆菌中发现并产生了新的甘氨酸,热甘氨酸和甲甘氨酸. 这些通过核糖体合成的具有独特的二硫化键和糖化,并确定了新的领先裂解机制.
科学领域:
- 生物化学和分子生物学
- 微生物学 微生物学
- 结构生物学 结构生物学
背景情况:
- 甘氨酸是一种通过O-和/或S-甘氨基化特征的核糖体合成和翻译后修饰 (RiPPs) 的类.
- 现有的关于糖生物合成和加工的知识是有限的,特别是在领导裂解机制方面.
- 鉴定新型RiPP扩大了我们对微生物自然产品多样性的理解.
研究的目的:
- 为了识别和描述来自Thermoanaerobacterium thermosaccharolyticum和Ornithinibacillus bavariensis的新型葡萄糖素.
- 阐明这些新型甘氨酸的生物合成途径,包括甘氨基化和领导加工.
- 为了研究特征化甘氨酸的结构特征和潜在的抗菌活性.
主要方法:
- 使用序列相似性网络进行生物信息分析,以识别假设的甘氨基转移酶和生物合成基因集群 (BGCs).
- 在大肠杆菌中BGCs的异质表达,用于生产热糖和糖.
- 使用质谱和NMR (隐含) 和AlphaFold3建模来预测裂变点的结构性表征.
- 实验证实了领导的裂变动机和C39酶活性.
主要成果:
- 在大肠杆菌中鉴定和异质生产热甘和甘.
- 结构性表征揭示了三种二硫化键和两种N-乙-葡萄糖胺 (GlcNAc) 部分在两种甘油素中.
- 一个新的Gly-Lys (GK) 动图被确定为由C39酶去除领导的裂解部位,与常见的双糖氨酸动图不同.
- 奥尼格利可辛显示出抗菌活性.
结论:
- 热甘和甘是甘家族的新成员,具有独特的结构特征.
- 这项研究揭示了RiPPs中领导处理的新型机制,涉及GK动机和PCAT.
- 这些发现有助于理解RiPP多样性,生物合成以及在抗微生物发现中的潜在应用.
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