耳鼻作为一种替代方法,用于在动物中注射大麻产品
Deborah da Costa Rodrigues1, Andrey Fabiano Lourenço de Aguiar1, Yolanda Paes Colli1
1Laboratório de Neuroquímica, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro.
Journal of visualized experiments : JoVE
|September 8, 2025
概括
一种使用大麻 (CBD) 丰富的大麻提取物在老鼠中的新型口腔粘膜药物递送方法被证明是安全有效的. 这种减压技术改变了海马中的关键蛋白质,表明神经疾病的治疗潜力.
科学领域:
- 神经科学是一个神经科学.
- 药理学 药理学是指药理学的学科.
- 动物科学动物科学
背景情况:
- 常规的口服药物通过管在动物中可以引起痛苦和发病率.
- 在临床前研究中,需要使用不那么侵袭和减轻压力的药物输送方法.
研究的目的:
- 为了在老鼠中探索加拿大大麻提取物 (CBD) 富含大麻的口腔/口腔给药.
- 评估这种新的输送方法的安全性和潜在的神经影响.
- 在动物福利方面,将这种方法与传统的食技术进行比较.
主要方法:
- 雄性大鼠每天接受CBD丰富的大麻提取物或对照剂 (中链甘油三) 的口服粘膜给药,持续15天.
- 治疗方法是使用微管管,轻轻地固定和亲切的触摸技术进行治疗.
- 剂量标准为3mg/kg/天的CBD,每天对体重进行监测以调整剂量.
主要成果:
- 在大鼠中,口腔粘膜注射含有CBD的大麻提取物被发现是安全的.
- 河马分析显示,GluA1和GFAP蛋白的表达有显著的变化.
- 这些变化表明质质受体功能的调节和神经炎症.
结论:
- 在动物研究中,口腔粘膜药物输送是一种可行的,非侵入性的,减压的替代品.
- 富含CBD的大麻提取物影响关键的神经通路,表明潜在的治疗应用.
- 这种方法可能有利于涉及谷氨基酶激发毒性和星病的疾病,改善研究中的动物福利.
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