赛斯氨酸γ酶缺失通过血栓素A2和神经性途径增强体洞膜收缩,而不会影响内皮功能
Tooyib A Azeez1, Clifford J Pierre1, Colin M Ihrig1
1Department of Health, Nutrition, and Food Sciences, Florida State University, Tallahassee, Florida, United States.
概括
在小鼠中,囊氨酸γ-酶 (CSE) 缺乏并没有损害内皮功能,但在勃起组织中增强了血管收缩. 这表明硫化 (H2S) 通过调节血管度来维持勃起功能至关重要.
科学领域:
- 生理学 生理学 生理学
- 生物化学 生物化学
- 血管生物学 血管生物学
背景情况:
- 硫化 (H2S) 是由囊氨酸γ-酶 (CSE) 生产的气体传递物,在血管功能中起作用.
- 内源H2S在勃起生理学中的特定作用尚不清楚.
- CSE淘汰赛 (CSE-KO) 鼠提供了一个模型来研究H2S缺乏对勃起功能的影响.
研究的目的:
- 研究CSE删除对小鼠血管和勃起组织反应性的影响.
- 为了确定CSE缺陷是否导致勃起组织内皮功能障碍.
- 阐明内源H2S在神经血管控制勃起功能中的作用.
主要方法:
- 从CSE-KO和野生型 (WT) 鼠身上对内动脉 (IIA),内动脉 (IPA) 和洞穴体 (CC) 的活体功能评估.
- 电线肌图和压力肌图用于测量血管收缩和放松.
- 评估内皮依赖和独立放松,以及非上腺,非胆上腺 (NANC) 神经介导反应.
主要成果:
- CSE-KO小鼠在CC中显示了受损的NANC神经介导放松.
- CSE-KO小鼠表现出对U-46619和电场刺激 (EFS) 的增强CC收缩,表明血管阻力增加.
- 在CSE-KO和WT小鼠之间没有观察到乙胆 (ACh),酸 (SNP) 或流中介放松的显著差异.
- 组织学分析显示没有显著的结构改造,但 IIA 和 IPA 中原蛋白增加的趋势.
结论:
- 慢性CSE缺乏症不会损害勃起组织中的内皮功能.
- CSE缺陷会改变神经性控制,并增加血管收缩敏感性,特别是在CC中.
- 由CSE产生的内源H2S对于调节勃起的神经血管控制和防止勃起组织中过度血管抵抗至关重要.
相关概念视频
Nitric Oxide Signaling Pathway
6.2K
Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure...
6.2K
Ligand-Gated Ion Channel Receptor: Gating Mechanism
3.8K
Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
3.8K
GPCRs Regulate Adenylyl Cylase Activity
7.3K
Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
7.3K
Male Sexual Response: Erection & Ejaculation
19.2K
Sexual stimulation can take various forms, such as physical touch and visual or auditory cues. When this happens, the parasympathetic reflex in the sacral portion of the spinal cord is activated. This reflex stimulates the release of nitric oxide (NO), which then dilates the arterioles in the penis, increasing blood flow to the erectile tissues - the corpora cavernosa and corpus spongiosum.
The blood filling the erectile tissues compresses the veins, which helps to prevent blood from leaving...
The blood filling the erectile tissues compresses the veins, which helps to prevent blood from leaving...
19.2K


