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High-Throughput Capable Three-Dimensional Tissue Model for Quantification of Electroporation Thresholds
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Published on: August 19, 2025

551

具有高通量能力的三维组织模型用于量化电穿孔值.

Robert H Williamson1, Alexia K Cash2, Anna E Riordan2

  • 1North Carolina State University; rhwilli4@ncsu.edu.

Journal of visualized experiments : JoVE
|September 8, 2025
PubMed
概括

本研究引入了一种高通量3D组织模型,以有效确定电穿孔值. 这种方法加速了用于基因疗法和癌症治疗等应用的电穿孔协议的表征.

科学领域:

  • 生物技术是生物技术.
  • 细胞生物学 细胞生物学
  • 医疗工程 医疗工程

背景情况:

  • 电穿孔使用电脉冲进行宏分子输送和组织切除.
  • 目前用于确定电穿孔值的方法是耗时的,并不完全代表体内条件.

研究的目的:

  • 开发和验证一个高通量3D组织培养模型,用于量化电穿孔值.
  • 为了提高在体内应用的电穿孔协议的表征效率.

主要方法:

  • 在3D组织模拟中利用非均的电场与HEK293细胞.
  • 使用环和针电极系统来输送GFP编码等离子体.
  • 通过光显微镜分析了转染细胞的空间分布,以获得值.

主要成果:

  • 在单个样本中成功确定了可逆和不可逆电穿孔值.
  • 证明了该模型能够有效地描述电穿孔协议的能力.
  • 与现有的值评估方法相比,实现了更高的吞吐量.

结论:

  • 开发的3D组织模型为评估电穿孔协议提供了高通量和更具体内代表性的方法.
  • 这种方法可以加速电穿孔的优化,用于治疗应用,包括遗传疾病治疗和癌症治疗.

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