雌性膜蛋白调节Drosophila melanogaster的交配后排卵,通过依赖于卵子的和独立于卵子的途径
Mengye Yang1, Melissa A White1, Geoffrey D Findlay2
1Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853.
概括
研究人员确定了七个关键的雌性蛋白调节排卵在果交配后,扩大对生殖过程的知识. 这些蛋白质,包括Lgr3和GabaβR1,突出了八胺 (OA) 神经元在控制这种必不可少的生物功能中的作用.
科学领域:
- 生殖生物学 生殖生物学
- 神经内分泌学神经内分泌学
- 进化生物学 进化生物学
背景情况:
- 排卵对于繁殖至关重要,在Drosophila melanogaster中,它增加了交配后的时间,由男性精液蛋白质ovulin触发.
- 八胺 (OA) 神经元信号传递对排卵调节至关重要,但女性控制的完整分子机制尚不清楚.
研究的目的:
- 通过使用卵子素作为分子探针,识别Drosophila melanogaster参与调节排卵的新型女性蛋白质.
- 阐明八胺 (OA) 神经元中已识别的蛋白质的作用及其在女性生殖道中的潜在功能.
主要方法:
- 采用进化速率共变和AlphaFold-Multimer预测来识别候选排卵调节蛋白.
- 对候选膜蛋白的敲除或突变进行了排卵测定.
- 在OA神经元和生殖道神经元中进行了特定组织的敲击实验.
主要成果:
- 确定了七种新的女性排卵调节剂:Lgr3,GabaβR1,SIFaR,mthl9,Smog,Cirl和CG6067.
- Lgr3和GabaβR1的功能依赖于卵蛋白,而SIFaR和mthl9的作用是独立的.
- 在OA神经元中需要Lgr3,GabaβR1,SIFaR和CG6067;Lgr3,GabaβR1,SIFaR,Smog和Cirl在生殖管内核化OA神经元中表达.
- 在Smog上有积极选择的证据,类似于卵子素的快速进化.
结论:
- 显著扩大了对Drosophila在交配诱导排卵的分子网络的理解.
- 突出了八胺 (OA) 神经元作为调节排卵的关键信号中心.
- 表明女性生殖道中已识别的蛋白质的潜在局部作用,并暗示对生殖蛋白质的进化压力.
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