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来自Grifola frondosa的多糖的制备和表征及其对人类肠道植物的调节作用

Meng-Ting Tang¹, Weiming Liu², Xingli Wang²

¹Key Laboratory for Food Microbial Technology of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, P. R. China.

Chemistry & biodiversity

|September 8, 2025

概括

提取了Grifola frondosa多糖 (GFPs) 并将其分成高分子量 (GFP-H) 和低分子量 (GFP-L) 分成. GFP-H更有效地调节了人类肠道微生物群,增加了有益的细菌和短链脂肪酸的产生.

科学领域:

食品科学与技术食品科学与技术
微生物学微生物学
生物化学生物化学

背景情况:

格里福拉果多糖 (GFPs) 是具有潜在健康益处的生物活性化合物.
了解GFP的结构功能关系对于其应用至关重要.
不同分子量 (Mw) 的GFPs对肠道微生物群的影响尚未得到充分证实.

研究的目的:

为Grifola frondosa多糖 (GFPs) 开发一种高效的提取方法.
研究不同MW GFPs对人类肠道微生物群的物理化学特性和体外影响.
确定Mw对GFPs肠道微生物群调节活性的影响.

主要方法:

开发了一种新的过氧化/酸辅助提取方法,用于GFPs.
使用超过方法将GFP分离成高分子量 (GFP-H) 和低分子量 (GFP-L) 分数.
通过模拟肠道发酵模型分析了物理化学特性 (形态学,学,热稳定性) 并评估了微生物群调节.

主要成果:

成功提取和分成GFPs;GFP-H (396.4 kDa) 和GFP-L (12.5 kDa) 的特征.
GFPs被肠道微生物降解,促进短链脂肪酸 (SCFA) 生产 (酸和黄油酸).
在促进有益细菌 (Bifidobacterium,Lactobacillus) 和抑制有害细菌 (Escherichia/Shigella) 方面,GFP-H表现出卓越的有效性.

结论:

分子重量显著影响Grifola frondosa多糖的肠道微生物群调节作用.
高MW GFPs显示了促进健康肠道微生物群的增强潜力.
这项研究为肠道健康应用中GFPs的结构-活性关系提供了宝贵的见解.

关键词:

格里福拉 (Grifola frondosa) 是一种多糖化合物.提取方法提取方法肠道菌群是指肠道上的植物.它具有调节效应的效果.

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