高血糖和ZIKV感染导致生理,免疫和氧化丸失调
Fernanda Carolina Ribeiro Dias1,2, Marcos de Lucca Moreira Gomes2, Katharina Medeiros Costa Gomes1
1Department of Veterinary Medicine, Federal Rural University of Pernambuco (UFRPE), Recife, Brazil.
概括
慢性高血糖和寨卡病毒 (ZIKV) 感染在小鼠中恶化了雄性生殖损伤. 糖尿病和ZIKV感染的结合严重损害了通过氧化应激和炎症的精子生成.
科学领域:
- 生殖生物学 生殖生物学
- 内分泌学 在内分泌学.
- 病毒学 病毒学
背景情况:
- 糖尿病 (DM) 患病率在全球范围内不断上升,特别是在发展中国家.
- 糖尿病患者在病毒感染期间经常经历恶化的症状,加速疾病的进展.
- 寨卡病毒 (ZIKV) 感染是一个严重的公共卫生问题.
研究的目的:
- 在小鼠模型中研究糖尿病和ZIKV感染对男性生殖参数的综合影响.
- 评估高血糖对ZIKV诱导的丸损伤的影响.
主要方法:
- 96只BALB/C小鼠被分为四组:对照组,糖尿病组,ZIKV感染组和糖尿病组加ZIKV感染组.
- 动物在感染后28天和42天 (dpi) 进行分析.
- 评估的参数包括身体和丸生物识别,乳酸脱酶,转激素,氧化应激标志物和细胞因子表达.
主要成果:
- 单独的ZIKV感染和糖尿病都会导致生殖损害.
- 与个体疾病相比,DM和ZIKV感染的组合显著加剧了丸损伤.
- 高血糖症延长了ZIKV感染和丸损伤的恶化,在糖尿病组中,一些参数未能恢复.
结论:
- 结合DM和ZIKV感染导致小鼠严重的生殖功能障碍.
- 慢性高血糖症加剧了ZIKV引起的丸损伤,并阻碍了恢复.
- 氧化应激和炎症是观察到的生殖损害的关键机制.
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