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Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Updated: Jun 19, 2026

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

可调节的细胞分离使用热响应的确定性横向位移装置.

Ze Jiang¹, Yusuke Kanno², Takasi Nisisako²

¹Department of Mechanical Engineering, School of Engineering, Institute of Science Tokyo, Tokyo 152-8550, Japan.

|September 9, 2025

概括

此摘要是机器生成的。

这项研究引入了一个温度控制的微流体芯片,用于可调节的细胞分离. 这种新的系统精确地从复杂的样本中分离出特定大小的细胞,增强生物医学研究和诊断.

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相关实验视频

Last Updated: Jun 19, 2026

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

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Published on: February 4, 2011

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Published on: September 3, 2013

A Microfluidic Technique to Probe Cell Deformability

A Microfluidic Technique to Probe Cell Deformability

Published on: September 3, 2014

科学领域:

生物医学工程生物医学工程
微流体学微流体学
细胞生物学细胞生物学

背景情况:

从异质样本中分离特定的细胞类型对于研究和诊断至关重要.
传统的确定性横移 (DLD) 系统具有固定的临界直径,限制了它们的灵活性.

研究的目的:

开发一种热响应的DLD微柱阵列,用于可调节的细胞分离.
使用温度控制来动态调节DLD系统的临界直径 (Dc).

主要方法:

在PDMS-微流体芯片中整合聚N-异烯胺 (PNIPAM) 水凝微柱.
使用佩尔蒂耶元件进行精确的温度调节 (20-40°C),以控制Dc (0.829.0μm).
用血样中使用MCF-7细胞进行模拟以确认热性能和实验验证.

主要成果:

通过温度控制证明可调节的细胞分离,具有可调节的临界直径.
在25°C (平均尺寸:17.6μm) 达到MCF-7细胞100%的纯度分离.
在26°C (平均大小:18.7μm) 进行了较大的MCF-7亚群的选择性分离,同时保持了细胞活力.

结论:

热响应的DLD平台可以从复杂的样本中精确,温度控制的细胞选择.
这项技术为推进基于细胞的诊断和生物医学研究提供了巨大的潜力.
该系统的可调性特性扩大了细胞隔离和分析中的应用.