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Updated: Jun 22, 2026

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
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可调节的pH开关的合理设计通过阴影链混合动力驱动的移位工程来实现

Xiaole Han1, Xiangyu Dong1, Xiaomei Lin1

  • 1Key Laboratory of Clinical Laboratory Diagnostics (Chinese Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, P. R. China.

Nucleic acids research
|September 9, 2025
PubMed
概括

这项研究介绍了影子链杂交启动的位移工程 (SHADE),这是一种基于DNA的方法,用于可编程和选择性的pH传感. 在酸性瘤微环境中,SHADE通过改善信号与噪声比提高了体内成像效果.

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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...