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Updated: Jan 6, 2026

Preparation of Non-human Primate Brain Tissue for Pre-embedding Immunohistochemistry and Electron Microscopy
11:55

Preparation of Non-human Primate Brain Tissue for Pre-embedding Immunohistochemistry and Electron Microscopy

Published on: April 3, 2017

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免疫电子显微镜:从样品制备到高分辨率成像的全面指南

Jinsai Wu1, Bo Su1, Leiyan Gu2

  • 1Histology and Imaging Platform, Core Facilities of West China Hospital, Chengdu, 610041, People's Republic of China.

Discover nano
|September 9, 2025
PubMed
概括

免疫电子显微镜 (IEM) 使用电子显微镜和免疫标记精确地定位生物分子. 这种技术对于理解细胞结构,疾病标志物和纳米医学等领域的相互作用至关重要.

关键词:
免疫电子显微镜的使用方法在嵌入后的嵌入后.预嵌入 预嵌入 预嵌入量化 量化 量化 量化这里是Tokuyasuasu.

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Miniaturized Sample Preparation for Transmission Electron Microscopy09:04

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To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
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The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
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Electron tomography can be performed either in TEM or STEM (scanning transmission...
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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
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