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在基工程中,Trichoderma harzianum的乙乙酶提高了热稳定性和效率

Ali Abolhasanzadeh Parizi1, Milad Lagzian1

  • 1Department of Biology, Faculty of Science, University of Sistan and Baluchestan, Zahedan, Iran.

Journal of biomolecular structure & dynamics
|September 9, 2025
PubMed
概括

计算方法设计了一种来自Trichoderma harzianum的热稳定的乙乙酶变体. 这种酶表现出增强的稳定性和活性,为纸和纸张生产提供了一个环保的替代品.

关键词:
热稳定的乙乙酶.催化效率提升 催化效率提升计算酶工程 计算酶工程在的方向进化.工业酶工业酶是什么稳定性改善 提高稳定性结构生物学结构生物学

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Acetoacetic ester synthesis is a method to obtain ketones from alkyl halides and β-keto esters. The reaction occurs in the presence of an alkoxide base that abstracts the acidic proton of the β-keto esters. The step results in an enolate ion which is doubly stabilized. The enolate then reacts with an alkyl halide via the SN2 process to produce an alkylated ester intermediate with a new C–C bond. The hydrolysis of the intermediate, followed by acidification, results in an...
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