基因组失衡调节了玉米中长非编码RNA的表达
Wenjie Yue1, Chuang Yu1, Hua Yang2
1College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China.
The Plant journal : for cell and molecular biology
|September 9, 2025
概括
基因组不平衡,或体积,显著影响基因表达,特别是长非编码RNAs (lncRNAs). 这项研究揭示了lncRNAs是剂量敏感的调节器,受基因组变化的影响比蛋白质编码基因更大.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 植物科学 植物科学
背景情况:
- 无倍体性 (个体染色体的增加或损失) 导致更大的基因组不稳定性比完整的染色体组的变化 (平倍体性/多倍体性).
- 基因组失衡会破坏宏分子静态测量,影响蛋白质编码基因 (PCG),微RNA和可转移元素 (TE).
- 长非编码RNAs (lncRNAs) 在对基因组失衡的反应中的作用在很大程度上是未知的.
研究的目的:
- 为了研究和比较无类与类/多类对玉米lncRNA表达的影响.
- 为了确定 lncRNAs 是否在基因组压力下作为剂量敏感调节剂.
主要方法:
- 用RNA测序 (RNA-seq) 来分析在不同种类的菌水平上的玉米成熟叶组织中的lncRNA表达.
- 分析包括比较相对于染色体剂量的 cis 定位和 trans 定位 lncRNA.
- 构建了cis-acting和trans-acting lncRNAs 的共同表达网络.
主要成果:
- 块化诱导了 cis 和转位 lncRNA 的显著调节, cis 作用的 lncRNA 从剂量补偿转向基因剂量效应.
- 转位的lncRNAs显示出各种反应,包括逆效应和与染色体剂量的正相关性.
- 与PCG相比,LncRNA对剂量变化表现出更大的敏感性,并显示出与TE相似的调制趋势.
- 排列性排列 (haploidy/polyploidy) 与无排列性排列性排列 (aneuploidy) 相比,导致 lncRNA 调制较少.
结论:
- 在基因失衡的背景下,LncRNAs是基因表达的敏感,剂量依赖的调节者.
- LncRNAs可能在调解积分症的生物后果方面发挥关键作用.
- 这项研究提供了对lncRNA功能及其在基因组压力下作为PCG调节者的潜力的见解.
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